Tissue Expression of SCNN1B Gene and Correlation Analysis Between Its SNPs and Eggshell Quality in Sansui Duck (Anas platyrhyncha domestica)
LIAO Chao-Mei, YOU Min-Fang, TAN Guang-Hui, LI Jie-Zhang, ZHANG Yi-Yu*
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education/College of Animal Science, Guizhou University, Guiyang 550025, China
Abstract：Sodium channel nonvoltage-gated 1β (SCNN1B) plays an important role in cation transport, which can transport Na+ and promote the secretion of Ca2+, thus affect the quality of eggshell. In this study, the egg duck variety Sansui duck (Anas platyrhyncha domestica) was used as the research object. The tissue expression of SCNN1B gene was detected by qRT-PCR, the genetic variation of SCNN1B gene was detected by direct sequencing, and the single factor analysis of variance (ANOVA) of SPSS 18.0 was used to analyze the effect of the variation on eggshell quality. The results showed that SCNN1B gene was expressed in different degrees in 12 tissues of Sansui duck, and the relative expression level was in the following order: uterus>kidney>leg muscle>duodenum>glandular stomach>chest muscle>liver>lung>brain>heart>spleen>muscular stomach, and the expression abundance of uterus was significantly higher than that of other tissues (P<0.05). Three moderately polymorphic loci were detected in SCNN1B gene, among which g.794619 C>T and g.794635 G>T were located in intron 8, and g.794564 G>A was a synonymous mutation in exon 8, which led to a change in the secondary structure of mRNA. The distribution of g.794635 G>T and g.794564 G>A genotypes deviated significantly from Hardy-Weinberg balance (P<0.01), and there was strong linkage disequilibrium among the 3 SNP loci, and there were 4 haplotypes and 5 diplotypes. The eggshell weight of individuals with TC genotype at g.794619 C>T was significantly higher than that of TT genotype (P<0.05), and that of H1H3 and H1H4 haplotypes was significantly higher than that of H1H1 (P<0.05). The results suggest that SCNN1B gene expression has tissue specificity in laying duck, the TC genotype at the locus of g.794619 C>T is beneficial to increase eggshell weight, and diplotype H1H3 is favorable to increase eggshell weight. This study provides a reference for the improvement of eggshell quality and the selection of genetic markers.
廖朝美, 游敏芳, 谭光辉, 李杰章, 张依裕. 三穗鸭SCNN1B基因组织表达及其SNPs与蛋壳品质的相关性分析[J]. 农业生物技术学报, 2021, 29(4): 723-731.
LIAO Chao-Mei, YOU Min-Fang, TAN Guang-Hui, LI Jie-Zhang, ZHANG Yi-Yu. Tissue Expression of SCNN1B Gene and Correlation Analysis Between Its SNPs and Eggshell Quality in Sansui Duck (Anas platyrhyncha domestica). 农业生物技术学报, 2021, 29(4): 723-731.
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