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2025年8月20日 星期三
农业生物技术学报  2021, Vol. 29 Issue (2): 288-297    DOI: 10.3969/j.issn.1674-7968.2021.02.009
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
基于RNA-seq技术挖掘杂交绵羊群体肌肉性状相关基因
史金平1, 马贵山2, 张全伟3, 刘婷1, 童建伟4, 孙鉴弘4, 魏玉兵4, 杨磊1, 成述儒1,*
1 甘肃农业大学 动物科学技术学院,兰州 730070;
2 甘肃省天祝藏族自治县农业农村局,武威 733299;
3 甘肃农业大学 生命科学技术学院,兰州 730070;
4 甘肃省张掖市甘州区平山湖蒙古族乡畜牧兽医站,张掖 734000
Identify the Genes Related to Muscle Traits in Crossbred Sheep Populations (Ovis aries) by RNA-seq Technology
SHI Jin-Ping1, MA Gui-Shan2, ZHANG Quan-Wei3, LIU Ting1, TONG Jian-Wei4, SUN Jian-Hong4, WEI Yu-Bing4, YANG Lei1, CHENG Shu-Ru1,*
1 College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2 Gansu Province Tianzhu Tibetan Autonomous County Agriculture and Rural Bureau, Wuwei 733299, China;
3 College of Life Science and Biotechnology, Gansu Agricultural University, Lanzhou 730070, China;
4 Livestock Station of Pingshan Lake Mongolian Township, Ganzhou, Zhangye City, Zhangye 734000, China
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摘要 肉质性能是影响绵羊(Ovis aries)养殖效益的主要经济性状,本研究旨在通过RNA-seq技术挖掘绵羊肉质品质相关基因,筛选优良商品代绵羊群体。以小尾寒羊(Small-tailed Han sheep, STH)母羊×萨福克(Suffolk sheep, SFK)公羊杂交一代(F1)商品代群体和小尾寒羊母羊(STH)×蒙古羊(Mongolian sheep, MG)公羊杂交一代(F1)商品代群体为研究对象,测定其屠宰性能和肉质品质,通过背最长肌转录组分析,挖掘影响绵羊肉质性状关键基因。结果显示,STH(♀)×SFK(♂)群体的屠宰性能和肉质品质均优于STH(♀)×MG (♂)群体。转录组结果显示,2个杂交群体背最长肌组织存在397个差异表达基因(differentially expressed genes, DEGs),主要富集在生长、发育等46个基因本体(Gene Ontology, GO)项和磷脂酰肌醇3-激酶(phosphatidyl inositol-3-kinase, PI3K)、蛋白激酶B (protein kinase B, Akt)所组成的信号通路PI3K/Akt、氨基酸合成等26条通路。根据功能和通路分析,筛选脂联素基因(adiponectin, ADIPOQ)、胰岛素样生长因子结合蛋白7基因(insulin-like growth factor binding protein 7, IGFBP7)、心脏α肌动蛋白基因(actin α1, cardiac muscle, ACTC1) 3个基因作为肉质性状相关的候选基因。qRT-PCR结果显示,STH (♀)×SFK (♂)群体背最长肌中ADIPOQIGFBP7相对表达量显著高于STH×MG群体(P<0.01),而ACTC1表达量显著低于STH (♀)×MG (♂)群体(P<0.01)。本研究表明,STH×SFK群体在屠宰性能、肉质品质方面均优于STH×MG群体,经济效益明显。筛选获得的差异表达基因可作为改良绵羊肉质性状的候选基因。
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作者相关文章
史金平
马贵山
张全伟
刘婷
童建伟
孙鉴弘
魏玉兵
杨磊
成述儒
关键词 绵羊比较转录组屠宰性能肉质品质商品代畜群    
Abstract:Meat quality is the main economic traits affecting the efficiency of sheep breeding. In order to select the high-efficiency commercial herds, transcriptome sequencing was applied for identifying the genes related to sheep growth performance and meat quality from the genetic level. The crossbred commercial generation herd (F1) of Small-tailed Han sheep ewe (STH)×Suffolk ram (SFK) and STH×Mongolian sheep ram (MG) was selected as the object. The slaughter performance and meat quality of these population were measured, meanwhile, transcriptome sequencing of longissimus dorsi muscle was also carried out for identifying the genes related to sheep growth performance and meat quality. The results showed that the production performance, slaughter performance and meat quality of STH (♀) × SFK (♂) generation was better than that of STH (♀) × MG (♂) generation. Transcriptomic results suggested that a total of 397 differentially expressed genes (DEGs) were identified in two generation. Most of these genes were mainly enrichmented in 46 Gene Ontology (GO) items such as growth and development and 26 pathways such as carbon metabolism, PI3K-Akt (phosphatidylinositide 3-kinases/protein kinase B), and amino acid synthesis. Finally, 3 genes of adiponectin (ADIPOQ), insulin-like growth factor binding protein 7 (IGFBP7) and actin α1 cardiac muscle(ACTC1) were taken as the candidate genes associated with meat quality traits according to results of function and pathway analysis. The results of qRT-PCR revealed that the relative expression of ADIPOQ and IGFBP7 in STH × SFK generation were significantly higher than that of STH (♀)×SFK (♂) generation (P<0.01), however the expression level of ACTC1 was significantly lower than that of STH (♀)×MG (♂) generation (P<0.01). These results indicated that the STH × SFK generation was superior to the STH×MG generation in slaughter performance and meat quality. The differentially expressed genes selected can be taken as candidate genes for improving the sheep meat quality.
Key wordsSheep    Comparative transcriptome    Slaughter performance    Meat quality    Commodity generation herd
收稿日期: 2020-05-18      出版日期: 2021-02-01
ZTFLH:  S826  
基金资助:甘肃省甘肃农业大学学科建设基金(GAU-XKJS-2018-024); 农业行业专项-牧区极端条件下牛羊应急专用饲料的开发与示范(201303062-5)
通讯作者: *chengsr@gsau.edu.cn   
引用本文:   
史金平, 马贵山, 张全伟, 刘婷, 童建伟, 孙鉴弘, 魏玉兵, 杨磊, 成述儒. 基于RNA-seq技术挖掘杂交绵羊群体肌肉性状相关基因[J]. 农业生物技术学报, 2021, 29(2): 288-297.
SHI Jin-Ping, MA Gui-Shan, ZHANG Quan-Wei, LIU Ting, TONG Jian-Wei, SUN Jian-Hong, WEI Yu-Bing, YANG Lei, CHENG Shu-Ru. Identify the Genes Related to Muscle Traits in Crossbred Sheep Populations (Ovis aries) by RNA-seq Technology. 农业生物技术学报, 2021, 29(2): 288-297.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2021.02.009     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2021/V29/I2/288
 
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