Cloning of ZlRPM1.1 and ZlRPM1.2 Genes and Responses to the Hyphal Infection of Ustilago esculenta in Jiaobai
YAN Mu-Xi*, CUI Hai-Feng*, LI Shuai, ZHAO Jin-Lan, YU Yue, ZHANG Ya-Fen, YU Xiao-Ping, YE Zi-Hong**
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine/College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Abstract：As an important aquatic vegetable in China, Jiaobai was the swollen stem of Zizania latifolia induced by hyphal infection of Ustilago esculenta. Resistance to pseudomonas maculicola 1 (RPM1), as a disease resistance related gene, can recognize the invasion signal of pathogens and activate the defense response of plants. In this study, 2 genes, ZlRPM1.1 (GenBank No. KP 729626) and ZlRPM1.2 (GenBank No. KP 729627) in Jiaobai responding to hyphal infection of U. esculenta were cloned by the methods of rapid amplification of cDNA ends (RACE). Alignment analysis of amino acid sequence predicted that the 2 genes belonged to CC-NBS-LRR (coiled coil-nucleotide binding site-leucine rich repeat) genes related to plant disease resistance, and were similar to the genes in Oryza sativa as high as 61% (ZlRPM1.1) and 79% (ZlRPM1.2), respectively. After artificial infection of U. esculenta in the plantlets of Z. latifolia, qPCR analysis of the stem showed that hyphae infection of U. esculenta could significantly induce the enhanced expression of ZlRPM1.1 and ZlRPM1.2 in Z. latifolia. The expression of the 2 ZlRPM1 genes among different swollen stems showed that the grey Jiaobai had significantly higher expression than that in normal Jiaobai, which might be related to the ability of hyphae infection between MT and T type of U. esculenta. During the development of swollen stem in normal Jiaobai, the expression of the 2 ZlRPM1 genes were significantly induced at the stem of mid- and late development stages. The highest expression was found at the swollen stem which was expanded to the length of 15 cm. There was significantly increased expression of ZlRPM1.2 gene in the initial swollen stem at the stage of 8-leaf, following with the higher expression in the stem than that in the leaf. However, no significant increased expression of ZlRPM1.1 was detected at initial stage of stem swelling, and the expression in the leaf was significantly higher than that in the stem. Above results indicate that both the 2 ZlRPM1 genes were involved in the formation of swollen stem induced by the infection of U. esculenta, and the ZlRPM1.2 gene might be an important regulatory gene involved in the response of stem in Z. latifolia to hyphal infection. The present study provides a reference for further investigation on the regulation mechanism of Z. latifolia plants in response to hyphal infection and stem swelling.
晏牧熙, 崔海峰, 李帅, 赵金兰, 虞悦, 张雅芬, 俞晓平, 叶子弘. 茭白ZlRPM1.1和ZlPRM1.2基因克隆及其对菰黑粉菌菌丝侵染的响应[J]. 农业生物技术学报, 2021, 29(11): 2061-2073.
YAN Mu-Xi, CUI Hai-Feng, LI Shuai, ZHAO Jin-Lan, YU Yue, ZHANG Ya-Fen, YU Xiao-Ping, YE Zi-Hong. Cloning of ZlRPM1.1 and ZlRPM1.2 Genes and Responses to the Hyphal Infection of Ustilago esculenta in Jiaobai. 农业生物技术学报, 2021, 29(11): 2061-2073.
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