Application of Multiplex Ligation-dependent Probe Amplification (MLPA) Assay in Pathogens Detection and Diagnosis of Diseases
WANG Yu-Meng*, ZHOU Ke*, SHAO Chun-Yan, YANG Yong-Chun, ZHOU Ying-Shan**, SONG Hou-Hui**
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province/Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology/College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China
Abstract：The multiplex ligation-dependent probe amplification (MLPA) technique is a sensitive technique for relative quantification of up to 50 different nucleic acid sequences in a single reaction. The MLPA procedure includes DNA denaturation, hybridization of probes and target DNA sequences, ligation of the probes, PCR amplification, capillary electrophoresis and data analysis. MLPA has a variety of applications including diagnosis and prognosis of tumors, detection of human genetic diseases, prenatal diagnosis. Due to the increasing occurrence of multiple infections and diseases, there is an urgent need to develop sensitive, specific, and high throughput tools to detect pathogens. MLPA is becoming a promising method in the area of high throughput detection of pathogens. This review gives an overview on the principles and procedures, applications in detection of pathogens and diagnosis of tumors and genetic diseases, advantages and limitations, and perspectives of this technique aiming to provide insights into the application and improvement of MLPA technique in animal pathogen detection and disease diagnosis.
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