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2025年8月11日 星期一
农业生物技术学报  2020, Vol. 28 Issue (8): 1410-1416    DOI: 10.3969/j.issn.1674-7968.2020.08.008
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
神经系统特异性转录因子基因NHLH2的5’UTR遗传变异与郏县红牛生长性状的相关分析
张婧敏1, 王艳红2, 宋成创2, 陈曦2, 张春雷2, 房兴堂2,*
1 上海农林职业技术学院,上海 201600;
2 江苏师范大学 生命科学学院,徐州 221116
Correlation Analysis between 5'UTR Genetic Variations of Nervous System-specific Transcription Factor Gene NHLH2 and Growth Traits in Jiaxian Red Cattle (Bos taurus)
ZHANG Jing-Min1, WANG Yan-Hong2, SONG Cheng-Chuang2, CHEN Xi2, ZHANG Chun-Lei2, FANG Xing-Tang2,*
1 Shanghai Vocational College of Agriculture and Forestry, Shanghai 201600, China;
2 School of Life Science, Jiangsu Normal University, Xuzhou 221116, China
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摘要 神经系统特异性转录因子NHLH2 (nescient helix-loop-helix 2)不仅调控脂肪组织中神经纤维的数量,而且对维持动物能量平衡具有重要作用。寻找与生长性状相关的DNA标记,可为肉牛(Bos taurus)遗传资源的保护、开发与利用提供科学依据。本研究利用PCR-单链构象多态性分析(PCR-single strand conformation polymorphism, PCR-SSCP)、DNA序列分析和生物信息学技术,探究郏县红牛(B. taurus) NHLH2基因5'UTR的遗传变异与其生长性状的相关性。结果显示,郏县红牛NHLH2基因5'UTR存在2个SNPs,即25G>C和179G>A,形成A和B 2个等位基因,等位基因频率分别为0.239和0.761;检测出AA、BB和AB 3种基因型,BB基因型为优势基因型。相关性分析表明,该基因3种不同基因型对郏县红牛的体重、体长和胸围有显著影响(P<0.05),且BB型个体的体重、体长和胸围均显著大于AA型(P<0.05)。上述实验结果证实,NHLH2基因5'UTR遗传突变与郏县红牛的生长性状相关,该位点可作为郏县红牛生长性状的分子标记。
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张婧敏
王艳红
宋成创
陈曦
张春雷
房兴堂
关键词 NHLH2(nescient helix-loop-helix 2)5'UTR遗传变异郏县红牛生长性状    
Abstract:Nescient helix-loop-helix 2 (NHLH2), as nervous system-specific transcription factor, can not only regulate the number of nerve fibers in adipose tissue, but also play an important role in maintaining the energy balance of animals. Searching for DNA markers related to growth traits can provide scientific basis for the protection, development and utilization of beef cattle (Bos taurus) genetic resources. In this study, the genetic variations in 5'UTR of NHLH2 gene and their association with growth traits in Jiaxian red cattle (B. taurus) were explored by the techniques of PCR-single strand conformation polymorphism (PCR-SSCP), DNA sequence analysis and bioinformatics. The 5'UTR of NHLH2 gene was amplified by PCR using the genomic DNA of 374 different individuals of Jiaxian red cattle as templates, and about 400 bp amplified products were obtained. It was found that the 5'UTR of NHLH2 gene was polymorphic by SSCP analysis. Three SSCP genotypes were identified and denominated as genotype AA, AB and BB, respectively. After sequencing the polymorphic products, it was found that there were 2 SNPs (25G>C and 179G>A) in the 5'UTR of NHLH2 gene, which constructed 2 alleles (A and B). A total of 133 amino acids were encoded in bovine NHLH2 gene. The result of homology analysis showed the high similarity of amino acid sequences among different species, which confirmed that the gene was very conservative in the evolution process. The linkage disequilibrium analysis of 2 SNPs showed that the linkage disequilibrium parameter r2 was 1, which indicated that the 2 SNPs were completely linked without cross or recombination. The result showed that there were 2 alleles A and B in the locus with corresponding frequencies of 0.239 and 0.761. And the frequencies of genotype AA, AB and BB were 0.139, 0.201 and 0.660, respectively. The frequency of allele B was higher than that of allele A. Allele B was the dominant allele, and genotype BB was the dominant genotype. The χ2-test value was 0.904, which suggested that the 5'UTR locus of NHLH2 gene was in Hardy-Weinberg equilibrium in this experimental population of Jiaxian red cattle. The polymorphism information content (PIC) value was 0.298, which indicated that the polymorphic locus belonged to moderate polymorphism. Using SPSS 19.0 software and the least square method, the correlation between different genotypes of polymorphic locus and growth traits in Jiaxian red cattle was analyzed. The polymorphisms for 5'UTR locus of NHLH2 gene had significant association (P<0.05) with chest circumference, body length and body weight, while had no significant association with other growth traits. The growth trait indexes of genotype BB individuals were significantly higher than those of genotype AA individuals (P<0.05). The above results confirmed that the correlation was detected between 5'UTR mutation of NHLH2 gene and the growth traits of Jiaxian red cattle, and the polymorphic locus could be used as a molecular marker for the growth traits, which could be used for improvement and breeding of Jiaxian red cattle.
Key wordsNHLH2 (nescient helix-loop-helix 2)    5'UTR    Genetic variation    Jiaxian red cattle    Growth trait
收稿日期: 2020-01-10     
ZTFLH:  S823.9+2  
基金资助:江苏省科技计划项目(SZ-XZ2017016;BN2016014);徐州市科技计划项目(KC18117;KC16NX073)
通讯作者: *xtfang@163.com   
引用本文:   
张婧敏, 王艳红, 宋成创, 陈曦, 张春雷, 房兴堂. 神经系统特异性转录因子基因NHLH2的5’UTR遗传变异与郏县红牛生长性状的相关分析[J]. 农业生物技术学报, 2020, 28(8): 1410-1416.
ZHANG Jing-Min, WANG Yan-Hong, SONG Cheng-Chuang, CHEN Xi, ZHANG Chun-Lei, FANG Xing-Tang. Correlation Analysis between 5'UTR Genetic Variations of Nervous System-specific Transcription Factor Gene NHLH2 and Growth Traits in Jiaxian Red Cattle (Bos taurus). 农业生物技术学报, 2020, 28(8): 1410-1416.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2020.08.008     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2020/V28/I8/1410
 
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