玉米<em>ZmPP2C3</em>基因的表达及互作蛋白分析

doi:10.3969/j.issn.1674-7968.2020.03.002

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摘要蛋白磷酸酶2C基因(protein phosphates 2C, PP2C)对植物生长发育和抵御逆境胁迫等方面具有重要的调控作用。本研究从前期玉米(Zea mays)干旱-复水转录组分析结果中筛选出一个编码PP2C蛋白的基因ZmPP2C3 (GenBank No. NM_001155565.2),序列分析显示ZmPP2C3基因的开放阅读框为1 227 bp,编码408个氨基酸,编码的蛋白分子量为43.28 kD,等电点为5.92;系统进化树分析显示该基因编码的蛋白序列与玉米中的PP2C蛋白AIB05995.1、AIG52088.1相似性最高,均为99.75%;亚细胞定位显示ZmPP2C3基因编码的蛋白位于细胞核中;qRT-PCR分析表明ZmPP2C3基因在玉米幼苗的根中表达量最高;在干旱、盐、高温及脱落酸(abscisic acid, ABA)处理下该基因均呈现出正向调控;酵母双杂结果显示ZmPP2C3蛋白与GRMZM2G180625、GRMZM2G038126、GRMZM2G155242、GRMZM2G110408、GRMZM2G069651存在相互作用。本研究为进一步分析ZmPP2C3的生物学功能提供了重要的理论依据。

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	王国瑞
	袁珍
	张鹏钰
	仇晓
	刘志学
	王同朝
	卫丽

关键词 ：玉米, 玉米蛋白磷酸酶2C3(ZmPP2C3), 非生物胁迫, 亚细胞定位, 蛋白互作

Abstract：Protein phosphates 2C (PP2C) genes play an important role in regulating plant growth and abiotic stress resistance. In this study, ZmPP2C3 (GenBank No. NM_001155565.2), screened from the previous drought-rewatering transcriptome analysis and encoding PP2C protein, was cloned in maize (Zea mays). Sequence analysis showed that the ORF of ZmPP2C3 gene was 1 227 bp, encoded 408 amino acids, and the molecular weight was 43.28 kD, isoelectric point was 5.92; Sequence alignment revealed that ZmPP2C3 shared high sequence homology with AIB05995.1 (99.75%) and AIG52088.1 (99.75%) in maize; Subcellular localization showed that the protein encoded by the ZmPP2C3 gene was detected in the nucleus. The qRT- PCR analysis showed that ZmPP2C3 gene had the highest expression in the root of corn seedlings, and the expression of ZmPP2C3 was positively regulated by drought, salt, high temperature and abscisic acid (ABA) treatment. Yeast two-hybrid analysis showed that ZmPP2C3 protein was interacted with GRMZM2G180625, GRMZM2G038126, GRMZM2G155242, GRMZM2G110408 and GRMZM2G069651. This study provides a reference for further biological function analysis of ZmPP2C3 gene.

Key words： Maize ZmPP2C3 Abiotic stress Subcellular localization Protein interaction

收稿日期: 2019-09-09

ZTFLH:

S513

基金资助:国家自然科学基金(31471452); 国家重点研究发展计划(2017YFD0301106)

通讯作者: **weili-wtc@126.com

作者简介: *同等贡献作者;

引用本文:

王国瑞, 袁珍, 张鹏钰, 仇晓, 刘志学, 王同朝, 卫丽. 玉米ZmPP2C3基因的表达及互作蛋白分析[J]. 农业生物技术学报, 2020, 28(3): 389-398.
WANG Guo-Rui, YUAN Zhen, ZHANG Peng-Yu, QIU Xiao, LIU Zhi-Xue, WANG Tong-Chao, WEI Li. Expression and Protein Interactions Analysis of ZmPP2C3 Gene in Maize (Zea mays). 农业生物技术学报, 2020, 28(3): 389-398.

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http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2020.03.002 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2020/V28/I3/389

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