Expression and Regulation Characteristics of Lipid Metabolism-related ACACA and FASN Genes in Laying Hens (Gallus gallus)
TIAN Wei-Hua, YANG Li-Yu, LI Hong, HAN Rui-Li, WANG Yan-Bin, TIAN Ya-Dong, KANG Xiang-Tao*, LIU Xiao-Jun*
College of Animal Science and Veterinary Medicine, Henan Agricultural University/Henan Innovative Engineering Research Center of Poultry Germplasm Resource/International Joint Research Laboratory for Poultry Breeding of Henan, Zhengzhou 450046, China
Abstract：AcetylCoA carboxylases alpha (ACACA) and fatty acid synthase (FASN), as crucial rate-limiting enzymes, exert significant roles in the biosynthesis of fatty acid and were regulated by multiple hormones in mammals. However, it is still unclear about the expression and regulation characteristics about ACACA and FASN genes in hen (Gallus gallus). The experiment was designed to explore the expression and regulation characteristics of ACACA and FASN genes related to lipid metabolism in hen. A total of 10 tissues including heart, liver, spleen, lung, kidney, pectorals, duodenum, abdominal fat, pancreas and ovary were obtained from 20- and 30-week-old Lushi green eggshell hens.In addition, more liver tissues were obtained from hens at different developmental stages including 5-, 15-, 20-, 30- and 35-week-old Lushi green eggshell hens. These tissues were used to detect the temporal and spatial expression pattern of ACACA and FASN genes via qRT-PCR. The hens and chicken embryonic primary hepatocytes (CEPHs) were treated with different concentrations of estrogen, and the regulatory mechanism of ACACA and FASN genes mediated by estrogen in vivo and in vitro were analyzed using qRT-PCR. The hens were fed with the basal diet supplemented with 4% or 8% soybean for 30 days, respectively, to study the effect of different doses of fat feed on ACACA and FASN genes expression. The results demonstrated that ACACA and FASN genes were expressed in all detected tissues including heart, liver, spleen, lung, kidney, pectorals, duodenum, abdominal fat, pancreas and ovary and shared a highest expression in livers. In addition, the expression levels of ACACA and FASN genes were highly significantly higher in the liver of peak-laying hens than that in the liver of pre-laying hens (P≤0.01). Moreover, compared to the control group, ACACA and FASN genes showed significantly or extremely significantly higher expression abundance in livers of hens treated with the 0.5, 1.0, 2.0 mg/kg dose of 17β-estradiol dissolved in olive oil or primary hepatocytes treated with either 25, 50 or 100 nmol/L dose of 17β-estradiol dissolved in ethyl alcohol (P≤0.05 or P≤0.01). Compared to the control group, ACACA and FASN genes were significantly (P≤0.05) and extremely significantly high (P≤0.01) in 8% soybean feed group compared to the control group, but no difference in 4% soybean feed group. Taken together, ACACA and FASN genes were likely to participate in the lipid metabolism in laying hen and their transcriptional expression were regulated via estrogen, and 8% soybean supplement in the basal diet contributes to an significant increase in their expression levels. This study paved the way for the further understanding the regulatory mechanism of ACACA and FASN genes on laying hen lipid metabolism.
田卫华, 杨丽, 玉李红, 韩瑞丽, 王彦彬, 田亚东, 康相涛, 刘小军. 蛋鸡脂肪代谢相关基因ACACA和FASN表达及调控特性[J]. 农业生物技术学报, 2019, 27(2): 280-288.
TIAN Wei-Hua, YANG Li-Yu, LI Hong, HAN Rui-Li, WANG Yan-Bin, TIAN Ya-Dong, KANG Xiang-Tao, LIU Xiao-Jun. Expression and Regulation Characteristics of Lipid Metabolism-related ACACA and FASN Genes in Laying Hens (Gallus gallus). 农业生物技术学报, 2019, 27(2): 280-288.
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