Abstract：Arabidopsis thaliana Ovate Family Proteins (AtOFPs) is a novel plant-specific transcription factor. As one of important member of the OFPs, AtOFP1 is the earliest and most studied transcriptional repressor. It has been proved that AtOFP1 can directly regulate the transcriptional activity of AtGA20ox, a key gene of GA synthase, and inhibit cell elongation. 3-Amino-acid loop extension homeodomain protein (TALE) plays a central role in plant development. It has a potential interaction with AtOFP1, and existing studies have shown that 9 OFPs and TALE proteins have a close functional association with their homeodomains. The literature showed that the ovate family proteins have potential interactions with TALE transcription factor, and identified 13 transcription factors that interact with AtOFP1 by the way of a screening library which include KNAT5. KNAT5 is expressed in the lateral root primordium and may participate in the occurrence of lateral roots, which has an important influence on plant growth and development. In order to further verify the interaction between AtOFP1 and KNAT5 and the effects of the interactions on Arabidopsis thaliana, we first verified the interaction between AtOFP1 and AtKNAT5 by yeast two-hybrid, then their biocompatibility was further verified by bimolecular fluorescence complementation (BiFC). In addition, the knat5 homozygous mutant was screened, and the gene expression of OFP1 and KNAT5 in Col-0, knat5 mutant, ofp1 mutant and HAOFP1 overexpression plant was analyzed by qRT-PCR to compare the expression pattern. Similar expression patterns in the roots and leaves of the plants were found. The existed synergistic expression in roots and leaves of Arabidopsis thaliana could affect the number of lateral roots and root length, which play an important role in plant growth and development. The present study lays the foundation for further exploring the mechanism of AtOFP1-KNAT5 interaction and its regulation function in plant growth and development.
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