Abstract：Bordetella bronchiseptica (Bb) is one of the pathogens of Swine infectious atrophic rhinitis (AR). Optimal conditions of Bb biofilm formation were developed, biofilm was well developed after 24 h, concentration of NaCl at 0.4%, pH 7.5, concentration of lactose at 0.5%. The protein expression profiles of whole Bb planktonic cells and biofilm-forming cells were investigated by comparative proteomics analysis, In this study, we compared the expression patterns of the whole Bb bacterial proteins originated from planktonic cells and biofilm-forming cells by a two-dimensional gel electrophoresis (2-DE). The results revealed that 15 protein spots were up-regulated and nine were down-regulated. Identified by mass spectrometry, the up-regulated protein spots mainly included elongation factor Tu (EF-Tu), superoxide dismutase (SOD) and molecular chaperone, involved in the stress, regulation and metabolism. The study is helpful to understand the Bb biofilm formation mechanism, and to prevent and control AR.
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