Abstract：Abstract Basic helix-loop-helix (bHLH) transcription factors play important roles in regulating plant growth-development and abiotic stress response. In order to enrich the research content of bHLH transcription factors and find new bHLH transcription factor in regulating secondary metabolite in Salvia miltiorrhiza, SmbHLH37 was cloned based on gDNA and cDNA. The gene sequence of SmbHLH37 was 1 506 bp with one intron of 27 bp, and 1 479 bp complete CDS (GenBank accession No. KP257470.1) encoding 492 amino acids. BlastP result showed that SmbHLH37 protein had high homology with bHLH3 in Sesamum indicum etc. Amino acid sequence analysis showed that SmbHLH37 contained a HLH domain and motif 7. Molecular evolutionary tree of SmbHLH37 protein and all the bHLH transcription factors in Arabidopsis thaliana indicated that SmbHLH37 protein was the closest relative to AtJAM3 (A. thaliana jasmonate-associated MYC2-like 3). Subcellular localization showed that SmbHLH37 protein was mainly localized in the nucleus. The expression pattern disclosed that the expression level of SmbHLH37 was the highest in leaves and the lowest in flowers. qRT-PCR analysis indicated that expression of SmbHLH37 could increase in a short time with 0.1 mmol/L abscisic acid (ABA) and wounding treatment. SmMYC2 and SmbHLH37 expression levels in SmMYC2 overexpressing transgenic S. miltiorrhiza lines OE-8 and OE-12 were detected, and the results showed SmMYC2 expression levels in OE-8 and OE-12 significantly or extremely significantly increased (P＜0.05 or P＜0.001). Meanwhile, the expression levels of SmbHLH37 in OE-8 and OE-12 were similar with SmMYC2 expression. The present data has laid a foundation for studying the function of SmbHLH37 in the future．
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