Pepper Nicotinamide Adenine Dinucleotide Phosphate Gene (NADPH) Transformation into Pepper and Transgenic Plants Identification of Resistance to Phytophthora capsici
Abstract：In order to analyze the relationship between the Pepper Nicotinamide Adenine Dinucleotide Phosphate Gene gene(canNADPH) and the resistance to Phytophthora capsici, make clear the role of canNADPH gene resistance to P. capsici, the pBI121-NADPH expression vector was constructed by the vector pBI121. Pepper (Capsicum annuum) susceptible cv. B12 was transformed with pBI121-NADPH by Agrobacterium tumefaciens-mediated transformation. Kanamycin-resistant regenerated lines were obtained, PCR and RT-PCR molecule detection confirmed that the five transgenic lines carried the target gene. The resistance of transgenic plants to P. capsici was identified by in vitro leaf technique. Results showed that the incidence of transgenic plants were reduced about 22.2% than those of the non-transgenic peppers, and the disease index of transgenic plants were also reduced 46.5%. The result shows that the canNADPH gene plays an important role at the resistance to P. capsici.
