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鉴别牛病毒性腹泻病毒基因型单克隆抗体的制备及其特性
徐树兰1,李少英2,冷雪2,张淑琴2,赵俊2,武华2
1. 中国农业科学院特产研究所
2.
Preparation and Preliminary Analysis of Monoclonal Antibodies against Different Genotypes of Bovine viral diarrhea virus
, , , , ,
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摘要 利用纯化的牛病毒性腹泻1型病毒(BVDV1)和2型病毒(BVDV2)为抗原免疫BALB/c小鼠,取脾细胞与SP2/0细胞融合,同时以BVDV1和BVDV2病毒作为包被抗原进行间接BVDV1-ELISA和BVDV2-ELISA检测,获得了3株单克隆抗体,其中1株是针对BVDV1的特异性抗体命名为BV1,另外1株是针对BVDV2的特异性抗体命名为BV2,第3株与BVDV1和BVDV2病毒都反应命名为BV12。交叉实验表明,3株单抗中仅有BV12与猪瘟病毒(Hog chorera virus,HCV)反应。BV1、BV2、BV12单抗腹水效价分别为105、106、105,均具有中和活性,中和效价最高达1:741455,分别属于鼠IgG1、IgG2a、IgG2a亚类,轻链均为?链。3株杂交瘤细胞的染色体数目分别为102、99、100条。
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徐树兰
李少英
冷雪
张淑琴
赵俊
武华
关键词 牛病毒性腹泻1型病毒牛病毒性腹泻2型病毒单克隆抗体    
Abstract:BALB/c mice were immunized by purified Bovine viral diarrhea virus-1(BVDV1)and Bovine viral diarrhea virus-2 (BVDV2). Murine myeloma SP2/0 cells were fused with the splenocytes of the immunized mice. Two indirect ELISA with BVDV1 and BVDV2 as coated antigen separately were used to screen antibody-producing hybridomas. Three McAbs against BVDV were obtained, one of them showed positive reaction only to BVDV1 named BV1; another showed positive reaction only to BVDV2 named BV2; the third one could react with both BVDV1 and BVDV2 named BV12. Cross reaction tests suggested that both BV1 and BV2 were specific to BVDV1 and BVDV2 respectively, BV12 was also reactive with Hog chorera virus (HCV) besides BVDV1 and BVDV2. The ascites titers of BV1、BV2、BV12 were 105、106、105 respectively, and all of them had neutralization activity, the highest neutralization titer was 1:741455. The subtypes of BV1、BV2、BV12 were IgG1、IgG2a、IgG2a respectively, the light chain of all McAbs was ????chain. The chromosome configuration of the three hybridoma cells were102、99、100 respectively.
Key wordsBovine viral diarrhea virus-1    Bovine viral diarrhea virus-2    monoclonal antibody
收稿日期: 2009-10-21     
通讯作者: 武华   
引用本文:   
徐树兰1,李少英2,冷雪2,张淑琴2,赵俊2,武华2. 鉴别牛病毒性腹泻病毒基因型单克隆抗体的制备及其特性[J]. , 2010, 18(5): 1001-1006.
, , , , ,. Preparation and Preliminary Analysis of Monoclonal Antibodies against Different Genotypes of Bovine viral diarrhea virus. , 2010, 18(5): 1001-1006.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2010/V18/I5/1001
 
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