Abstract：Abstract object To prepare the rapid diagnostic kit of H5 subtype avian influenza virus (AIV-H5) and set up AIV-H5 rapid diagnostic technology. Methods Balb/c mice were immunized by purified antigen, to produce two good matching monoclonal antibodies (McAb) against AIV-H5. It’s to develop the rapid diagnostic kit using gold immunochromatography, and evaluate its capability. Result The kit has high sensitivity, specificity and good stability. Detecting the man-made infected chicken tissues , the kit’s detecting result was as same as embryo culture methods. Contrasting the results of filed experiments with fluorescent RT-PCR, the coincidence rate was 100%. Conclusion The rapid diagnostic kit of AIV-H5 is a pecical , sensitive and rapid production for AIV-H5 detection.
