Abstract：Sequencing, PCR-RFLP and dCAPS methods were used to study the polymorphism in exon 3 and 3′UTR of B-cell translation gene 2 (Btg2), which was associated with the multi-vertebra traits of Hebei Small Tail Han Sheep (Ovis aries). 3 SNPs (A150G, C243T and A607G) were found in the amplified fragments. The restriction endonuclease digestion analysis was used to determine genotypes and then haplotype analysis was conducted. The results of the independence Chi-square test showed that genotype frequencies at Site 150 had no significant difference among different vertebral patterning sheep (P>0.05). And at Site 243, the genotype frequencies had extremely significant difference among different vertebral patterning sheep (P<0.05), while at Site 607, the result was of significant difference. The result of haplotype frequencies among different phenotypes showed a significant difference as well by independence Chi-square test (P<0.05). These results implied that Site 243, Site 607 and GTA haplotype may have associations with the multi-vertebrae traits of Hebei Small Tail Han Sheep.
