Abstract:Markers linked to Watermelon mosaic virus ( WMV ) resistant gene in cucumber (Cucumis sativus) were identified using 120 recombinant inbreed lines (RIL6) made between a resistant and susceptible parent (Qiupeng× No.8 European). Genetic analysis based on virus inoculation at the seedling stage showed that the resistant trait in Qiupeng was controlled by a single recessive locus. The AFLP marker E-ACT/M-CTT-427 was linked to the resistant gene with a genetic distance of 8 cM. The polymorphism came from a 6 bp deletion of poly(T) repeat in the susceptible parent. And this AFLP marker was converted into a co-dominant sequence-characterized amplified region(SCAR) marker which could be useful in marker-assisted breeding for WMV resistance.