Abstract:Protein content in meal, hull content, oil content in hull, 1000-seed weight and seed oil content were analyzed using 132 F2:3 families which derived from across between yellow-seeded parent GH06 and black-seeded parent P174 of Brassica napus. The results showed that these five traits were typical quantitative trait with the phenotype continual distribution. Using the method of composite interval mapping(CIM), a total of 13 QTL(quantitative trait locus) affecting these five traits were detected in linkage group 7, 8, 13, 14, 19, 20 and 21. As for seed oil content and 1000-seed weight, only one QTL was obtained with 14.1% and 18.7% contribution for the phenotype variation respectively. Five QTL, 3 QTL and 3 QTL were detected for the protein content in meal, hull rate and oil content in hull and the contribution for each trait ranged from 7.8%~14.4%, 26.5%~32.8% and 8.3%~12.1% respectively. Furthermore, overlapping region was observed in linkage 20 among the QTL of protein content in meal, oil content in hull and 1000-seed weight.