Pathogenicity Analysis of Papaya leaf curl Guangdong virus and Euphorbia leaf curl virus
LI Jing-Yuan1, LIN Wen-Zhong1, LI Ding-Shan1, MA Chong-Huan1, WU Zu-Jian1, DU Zhen-Guo1, ZHANG Jie1,2,*
1 Institute of Plant Virology/State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, China
Abstract：In recent years, Papaya leaf curl Guangdong virus (PaLCuGDV) and Euphorbia leaf curl virus (EuLCV) were usually identified in Passiflora edulis. The full-length sequences of the 2 viruses, 2 732 and 2 745 bp respectively, were amplified from Passiflora edulis showing typical geminiviral damage symptoms in Fujian province. In order to determine the pathogenicity of PaLCuGDV and EuLCV, the infectious clones of 2 viruses were constructed by homologous recombination technology and Nicotiana benthamiana plants were inoculated with Agrobacterium-mediated method. The results showed that the symptoms of N. benthamiana inoculated with EuLCV were obviously dwarfed and the top leaves were deformed and curled, which was significantly heavier than that of PaLCuGDV, while the latter only showed slightly curly new leaves and yellowing leaves. Further more, the symptoms of mixed infection of the 2 viruses was much heavier than any of the symptoms induced by PaLCuGDV or EuLCV alone. Southern blot results showed that N. benthamiana inoculated with PaLCuGDV and EuLCV alone or in combination had high virus accumulation. In this study, the infectious clone of PaLCuGDV was constructed for the first time, and it was found that the mixed infection of EuLCV and PaLCuGDV could aggravate the induced symptoms. This study provides a reference for understanding the pathogenic mechanism of PaLCuGDV and EuLCV.
[1] 顾珠琳, 王健华, 张树珍, 等. 2022. 海南省西番莲广东番木瓜曲叶病毒的检测鉴定[J]. 南方园艺, 33(2): 9-14.
(Gu Z L, Wang J H, Zhang S Z, et al.2022. Detection and identification of passionflower Papaya leaf curl Guangdong virus in Hainan province[J]. Southern Horticulture, 33(2): 9-14.)
[2] 黄爱军, 王莹, 胡笑林. 2019. 西番莲病毒病研究进展[J]. 中国南方果树, 48(3): 141-146.
(Huang A J, Wang Y, Hu X L.2019. Research progress on viruses of passionfruit[J]. South China Fruit, 48(3): 141-146.)
[3] 严佳文, 袁启凤, 彭志军,等. 2018. 西番莲病毒病害研究进展[J]. 热带农业科学, 38(4): 85-94.
(Yan J W, Yuan Q F, Peng Z J, et al.2018. Research progress on viruses of passionfruit[J]. Chinese Journal of Tropical Agriculture, 38(4): 85-94.)
[4] 李刚, 赵黎明, 高颖, 等. 2014. 侵染一品红的一品红曲叶病毒山东分离物的全基因组克隆和序列分析[J]. 植物保护, 40(3): 133-137.
(Li G, Zhao L M, Gao Y, et al.2014. Molecular detection and sequence analysis of Euphorbia leaf curl virus infecting Euphorbia in Shandong province[J]. Plant Protection, 40(3): 133-137.)
[5] 李战彪, 谢慧婷, 陈锦清, 等. 2022. 侵染广西西番莲的双生病毒基因组克隆与序列分析[J]. 植物病理学报, 52(4): 613-620.
(Li Z B, Xie H T, Chen J Q, et al.2022. Detection and sequence analysis of begomoviruses from Passiflora caerulea L. in Guangxi, China[J]. Acta Phytopathologica Sinica, 52(4): 613-620.)
[6] 谢丽雪, 张小艳, 郑姗, 等. 2019. 福建西番莲上广东番木瓜曲叶病毒的分子鉴定[J]. 东南园艺, 7(5): 19-22.
(Xie L X, Zhang X Y, Zheng S, et al.2019. Molecular identification of Papaya leaf curl Guandong virus infecting passion fruit in Fujian province[J]. Southeast Horticulture, 7(5): 19-22.)
[7] 俞超维. 2020. 中国百香果病毒病调查鉴定及抗病相关基因的解析[D]. 博士学位论文, 福建农林大学, 导师: 吴祖建, pp. 3-47.
(Yu C W.2020. Investigation and identification of viral diseases on Passiflora edulis in China and mining of resistance related genes of Passiflora edulis[D]. Thesis for Ph. D., Fujian Agriculture and Forestry University, Supervisor: Wu Z J, pp. 3-47.)
[8] 张鲁斌, 周国辉, 李华平, 等. 2005. 侵染广州番木瓜的曲叶病毒DNA-A分子特征及生物学测定[J]. 中国农业科学, 38(9): 1805-1810.
(Zhang L B, Zhou G H, Li H P, et al.2005. Molecular characterization of Papaya leaf curl virus infecting Carica papaya in Guangzhou and its biological test[J]. Scientia Agricultura Sinica, 38(9): 1805-1810.)
[9] Bennett A, Agbandje M M.2020. Geminivirus structure and assembly[J]. Advances in Virus Research, 108: 1-32.
[10] Briddon R W, Bull S E, Mansoor S.et al.2002. Universal primers for the PCR-mediated amplification of DNA β[J]. Molecular Biotechnology, 20(3): 315-318.
[11] Cerqueira-Silva C B M, Jesus O N, Santos E S L, et al.2014. Genetic breeding and diversity of the genus Passiflora: Progress and perspectives in molecular and genetic studies[J]. International Journal of Molecular Sciences, 15(8): 14122-14152.
[12] Cheng Y H, Deng T C, Chen C C.2014. First report of Euphorbia leaf curl virus and Papaya leaf curl Guangdong virus on passion fruit in Taiwan[J]. Plant Disease, 98(12): 1746.
[13] Cota-Sánchez J H, Remarchuk K, Ubayasena K.2006. Ready-to-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue[J]. Plant Molecular Biology Reporter, 24(2): 161-167.
[14] Fiallo-Olivé E, Pan L L, Liu S S, et al.2020. Transmission of begomoviruses and other whitefly-borne viruses: Dependence on the vector species[J]. Phytopathology, 110(1): 10-17.
[15] Gioria R, Espinha L M, Rezende J A M, et al.2002. Limited movement of Cucumber mosaic virus (CMV) in yellow passion flower in Brazil[J]. Plant Pathology, 51(2): 127-133.
[16] He Z F, Yu H, Luo F F.2004. Detection of whitefly-transmitted geminiviruses from tomato by PCR[J]. Virologica Sinica, 19(1): 67-69.
[17] Kil E J, Seo H, Byun H S, et al.2016. First report of Euphorbia leaf curl virus in passion fruits in South Korea and its natural occurrence in papaya[J]. Plant Disease, 100(4): 865-865.
[18] Ma X Y, Cai J H, Li G X, et al.2004. Molecular characterization of a distinct begomovirus infecting Euphorbia pulcherrima in China[J]. Journal of Phytopathology, 152(4): 215-218
[19] Manicom B, Ruggiero C, Ploetz R C, et al.2003. Diseases of passion fruit[J]. Diseases of Tropical Fruit Crops, 52(11): 1969-1970.
[20] Nawaz-ul-Rehman M S, Fauquet C M.2009. Evolution of geminiviruses and their satellites[J]. FEBS Letters, 583(12): 1825-1832.
[21] Stanley J.2004. Subviral DNAs associated with geminivirus disease complexes[J]. Veterinary Microbiology, 98(2): 121-129.
[22] Wang X Y, Xie Y, Zhou X P.2004. Molecular characterization of two distinct begomoviruses from papaya in China[J]. Virus Genes, 29(3): 303-309.
[23] Wyatt S D, Brown J K.1996. Detection of subgroup Ⅲ geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction[J]. Phytopathology, 86(12): 1288-1293.
[24] Wu J, Zulfiqar A, Huang C.2011. Infectivity of Euphorbia leaf curl virus and interaction with Tomato yellow leaf curl China betasatellite[J]. Archives of Virology, 156(3): 517-521.
[25] Yang C X, Zheng L M, Wu Z J, et al.2013. Papaya leaf curl Guangdong virus and Ageratum yellow vein virus associated with leaf curl disease of tobacco in China[J]. Journal of Phytopathology, 161: 201-204.
[26] Ye T, Zheng G H, Guo Y, et al.2022. First report of Euphorbia leaf curl virus infecting Passiflora edulis in Fujian province, China[J]. Journal of Plant Pathology, 104(2): 807-807.
[27] Zerbini F M, Briddon R W, Idris A, et al.2017. ICTV virus taxonomy profile: Geminiviridae[J]. Journal of General Virology, 98(2): 131-133.
[28] Zhang J, Cui G J, Yang C X, et al.2014. First report of Ageratum yellow vein virus and Papaya leaf curl Guangdong virus on Euphorbia pulcherrima (poinsettia) in China[J]. Journal of Plant Pathology, 96(4, Supplement): 128.
