Effects of Transport Stress on Apoptosis and Expression of Bcl-2 and Bax in the Immune Organs of Goat (Capra hircus)
PENG Rui-Ni1, LIU Ben2,3, YU Si-Jiu1,*
1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2 College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China; 3 Jiangxi Lvke Agriculture and Animal Husbandry Technology Co., Ltd., Yichun 336000, China
Abstract：Apoptosis is an important way to maintain the homeostasis and function of immune organs, which is beneficial to maintain homeostasis in goats (Capra hircus) under transport stress. The study was to investigate the effects of transport stress on apoptosis and the expression of B-cell lymphocytoma-2 (Bcl-2) and Bcl-2 related X protein (Bax) in goats immune organs. Twelve healthy male Ganxi goats were randomly divided into 3 groups: Control group (non transported), transport 2 h group and transport 6 h group. The immune organs (spleen, mesenteric lymph nodes) of goats were collected after transportation. Terminal dexynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to detect apoptosis in immune organs. Immunohistochemistry, Western blot, qPCR methods were used to locate and detect at the protein and gene level. TUNEL analysis showed that the apoptosis rate of spleen and lymph nodes in transport 2 and 6 h were significantly higher than the control group (P<0.01); Immunohistochemical results showed that Bax and Bcl-2 were positively expressed in goats splenic nodules and marginal area of spleen, and lymphatic nodule and surrounding diffuse lymphatic tissue of lymph nodes before and after transportation, but the expression intensity varied among the 3 groups. Western blot results showed that the proteins expression levels of Bax, Bcl-2 and Bax/Bcl-2 ratio were not statistically different among the 3 groups in spleen and lymph nodes (P>0.05). qPCR results showed that, compared with the control group, the mRNA expression levels of Bax and Bcl-2 in transport 2 and 6 h groups and Bax/Bcl-2 ratio in transport 6 h group were significantly increased in spleen. In the lymph nodes, the gene expression levels of Bax and Bcl-2 in transport 2 h group were significantly decreased (P<0.01), and the gene expression of Bcl-2 in 6 h group was significantly increased (P<0.01), Bax/Bcl-2 ratio in transport 2 and 6 h groups was significantly decreased (P<0.01). The results showed that transport stress could lead to increase apoptosis and significant change of the expression of Bax and Bcl-2 in immune organs. The present study provides a theoretical basis for preventing and treating the effects of transport stress in goats.
彭瑞妮, 刘犇, 余四九. 运输应激对山羊免疫器官细胞凋亡及Bcl-2和Bax表达的影响[J]. 农业生物技术学报, 2022, 30(4): 676-685.
PENG Rui-Ni, LIU Ben, YU Si-Jiu. Effects of Transport Stress on Apoptosis and Expression of Bcl-2 and Bax in the Immune Organs of Goat (Capra hircus). 农业生物技术学报, 2022, 30(4): 676-685.
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