Dissecting the Genetic Basis of Stem Length and Stem Diameter in Maize (Zea mays) Using a Maize-teosinte Introgression Line Population
LI Fang1, LIU Zhi-Hua1, HU Jin-Xiang1, XIAO Ren-Jie1, ZHU Xue-Qing1, XU Ying1, DENG Min1,2, LI Rui-Lian1,2, LUO Hong-Bing1,2, HUANG Cheng1,2,3,*
1 College of Agronomy, Hunan Agricultural University, Changsha 410128, China; 2 Maize Engineering Technology Research Center of Hunan Province, Changsha 410128, China; 3 Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Changsha 410128, China
Abstract：Stem length and stem diameter, as two important traits of maize (Zea mays) stem, are closely related to maize lodging resistance, also directly or indirectly affect maize yield and plant architecture, and they are important target traits in maize breeding. This study performed a high-resolution QTL mapping for stem length and stem diameter using the multiple QTL model implemented in R/qtl, together with 19 838 SNP markers. The introgression line population of 866 lines was derived from a cross between a typical maize inbred line W22 and a typical accession of teosinte. The results showed that there were extensive genetic variations in stem length and stem diameter, which were typical quantitative traits controlled by multiple micro-effect genes. A total of 2 QTLs controlling stem length was located on chromosome 1 and 4 respectively, with the phenotypic contribution rate of 4.51% and 2.68%, and the additive effect of 0.41 and 0.39 cm, respectively. In addition, 5 QTLs controlling stem diameter were located on chromosome 1, 2, 5, 6 and 7, respectively, and the phenotypic contribution rate of each QTL varied from 2.06% to 8.32%, and the additive effect varied from 0.03 to 0.09 cm. Further analysis of candidate genes for the largest effect QTL (qSD5-1) of stem diameter, 13 possible candidate genes were screened out. This study might provide a theoretical basis for elucidating the genetic basis of stem length and stem diameter, the cloning of related genes and molecular marker-assisted selection breeding in maize.
李芳, 柳志华, 胡锦祥, 肖仁杰, 朱雪晴, 徐莹, 邓敏, 李瑞莲, 罗红兵, 黄成. 利用玉米-大刍草渗入系群体解析玉米茎长和茎粗的遗传基础[J]. 农业生物技术学报, 2021, 29(2): 216-223.
LI Fang, LIU Zhi-Hua, HU Jin-Xiang, XIAO Ren-Jie, ZHU Xue-Qing, XU Ying, DENG Min, LI Rui-Lian, LUO Hong-Bing, HUANG Cheng. Dissecting the Genetic Basis of Stem Length and Stem Diameter in Maize (Zea mays) Using a Maize-teosinte Introgression Line Population. 农业生物技术学报, 2021, 29(2): 216-223.
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