Analysis of Gene Expression Profiles of Rice (Oryza sativa) Monogenic Line of IRBL9-W During Its Early Stage of Resistance to Magnaporthe oryzae
JIANG Zhao-Yuan1, 2, REN Jin-Ping1, 2, *, LI Li1, 2, LIU Xiao-Mei1, 2, *, ZHU Feng1, 2, WANG Ji-Chun1, 2, SUN Hui1, 2
1 Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Changchun 130033, China; 2 Key Laboratory of Integrated Pest Management on Crops in Northeast, Ministry of Agriculture, Gongzhuling136100, China
Abstract：The rice (Oryza sativa) monogenic line of 'IRBL9-W' (containing resistance gene (Pi9)) was resistant to most of the Magnaporthe grisea isolates collected in Jilin province, indicating that the isolates contain the avirulence gene (AVR) widely spread in Jilin Providence. To investigate the gene expression profiles of the rice during the early interaction between Pi9 and avirulence gene (AVR-Pi9), the rice monogenic line of 'IRBL9-W' (Pi9) inoculated with Magnaporthe grisea isolate C2 (AVR-Pi9) in this study. The RNA-seq technology was used to analyze the expression profiles of the inoculated and uninoculated rice leaves of 'IRBL9-W' (Pi9) at 36 h after inoculation and a total of 2 311 genes were found to express differently in the two samples. The analyses of GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways showed that 3 of the 4 leucine zipper protein genes were up-regulated, 1 zinc finger protein gene was up-regulated and all the WRKY protein genes were down-regulated among transcriptional regulator genes, 2 chitinase genes were up-regulated among the10 chitinase genes and all the two β-1,3 glucanase genes were down-regulated among the fungal cell wall degrading enzyme genes, 1 pathogenesis-related protein 1 gene (PR1) was up-regulated and the other genes were down-regulated in the plant-pathogen interaction pathway, all of the differentially expressed genes were up-regulated in the flavonoid biosynthesis pathway, and 9 genes were up-regulated and 1 gene was down-regulated in the phenylpropanoid biosynthesis pathway. The differentially expressed gene products in the 3 pathways were involved in getting the reactive oxygen species, the hypersensitive response, and the antioxidant process. The functions of the compounds relating to these gene products were antioxidation, antifungal action, and lignification. This study would provide a theory to further understand the mechanism of rice blast resistance.
姜兆远, 任金平, 李莉, 刘晓梅, 朱峰, 王继春, 孙辉. 水稻单基因系品种IRBL9-W抗瘟初期基因表达谱分析[J]. 农业生物技术学报, 2020, 28(9): 1662-1674.
JIANG Zhao-Yuan, REN Jin-Ping, LI Li, LIU Xiao-Mei, ZHU Feng, WANG Ji-Chun, SUN Hui. Analysis of Gene Expression Profiles of Rice (Oryza sativa) Monogenic Line of IRBL9-W During Its Early Stage of Resistance to Magnaporthe oryzae. 农业生物技术学报, 2020, 28(9): 1662-1674.
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