玉米RIL群体SNP偏分离分析

doi:10.3969/j.issn.1674-7968.2019.09.003

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摘要偏分离是自然界普遍存在的现象,是生物进化的重要动力。随着分子标记技术的发展进步,不同作物的遗传研究中报道了大量偏分离现象,偏分离的比例和遗传效应因物种、群体、标记类型等的不同而变化,并存在偏分离热点区域。为探讨玉米(Zea mays)偏分离现象及其产生原因,本研究以热带玉米自交系B31-3与温带玉米自交系HZ4杂交得到的F₈代重组自交系(recombinant inbred lines, RIL)群体为材料,共198份株系,采用MaizeSNP3072芯片对该重组自交系群体进行基因型分析,获得747个在双亲间有多态性的SNP数据,通过偏分离分析发现,有279个SNP标记发生偏分离(P<0.05),占总标记数的37.3%。其中,偏向母本B31-3的SNP有194个,占偏分离SNP的69.5%;偏向父本HZ4的SNP有85个,占偏分离SNP的30.5%。在玉米6条染色体上共发现10个偏分离热点区域。10个偏分离热点区域(segregation distortion regions, SDRs)中仅有3个偏向父本HZ4,另外7个全部偏向母本B31-3。这些偏分离的发生及偏分离热点区域的形成可能与配子体选择及高温环境有关。本研究为探究玉米偏分离规律提供了基础资料。

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	付忠军
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	李淑君
	陈荣丽
	祁志云
	杨华

关键词 ：玉米, 偏分离, SNP, 重组自交系(RIL)

Abstract：Segregation distortion is an ubiquitous phenomenon in nature and an important driving force for biological evolution. With the development and progress of molecular marker technology, a large number of segregation distortion phenomena are reported in the genetic research of different crops and the proportion of marker segregation distortion and genetic effect vary according to species, population, marker type, etc., while there were segregation distortion regions (SDRs). In order to investigate the phenomenon of maize (Zea mays) segregation distortion and its causes, a F₈ population consisting of 198 recombinant inbred lines (RIL) was used in the study, which derived from a cross between a tropical maize inbred line B31-3 and a temperate maize inbred line HZ4. A total of 747 SNPs, covering 10 maize chromosomes, were detected with polymorphism between parent B31-3 and HZ4. Segregation distortion analysis was performed among the 747 SNPs for the RIL population. The results of chi-square test (χ²=96.92, >χ²_0.05=6.63)(P<0.05), showed that the distribution proportion of the female B31-3 and male HZ4 genotypes deviated from the theoretical ratio of 1∶1 in RIL population and the genetic contribution rate of female B31-3 to offspring was higher than that of male HZ4. The result indicated that 279 SNPs (37.3%) showed the genetic distortion (P<0.05). Of the total segregation distortion SNPs, 194 SNPs (69.5%) deviated toward female parent B31-3, while 85 SNPs (30.5%) deviated toward male parent HZ4. Totally, 10 SDRs were detected among 6 chromosomes. 3 SDRs were distorted to HZ4, while 7 SDRs were skewed to B31-3, which showed female parent gametophytes had an competitive advantage in forming a zygote in this study. There were 6~64 segregation distortion SNPs in SDRs and the span of SDRs were 3.69~78.11 cM. SDR5-1 and SDR5-3, detected in this study, were located in near regions where gametophyte genes related to segregation distortion had been reported. Additionally, in the study, the RIL population was affected by high temperature in the group building process. So, the occurrence of segregation distortion and the formation of SDRs might be related to gametic selection and high temperature environment in this study. This study provides basic data for understanding the law of segregation distortion of maize.

Key words： Maize Segregation distortion SNP Recombinant inbred lines (RIL)

收稿日期: 2019-01-14

ZTFLH:

S513

基金资助:国家重点研发计划(No. 2016YFD03003)、重庆市科委社会事业与民生保障科技创新专项(No. cstc2016shms-ztzx80015; No. cstc2016shms-ztzx80013)、重庆市农业科学院创新团队项目(No. NKY-2018QC02)和重庆市科研机构绩效激励引导专项(No. cstc2018jxjl80034)

通讯作者: qzy5609@163.com;cqyangh@163.com

引用本文:

付忠军, 官玲, 张丕辉, 董昕, 李淑君, 陈荣丽, 祁志云, 杨华. 玉米RIL群体SNP偏分离分析[J]. 农业生物技术学报, 2019, 27(9): 1542-1549.
FU Zhong-Jun, GUAN Ling, ZHANG Pi-Hui, DONG Xin, LI Shu-Jun, CHEN Rong-Li, QI Zhi-Yun, YANG Hua. Segregation Distortion Analysis on Maize (Zea mays) RIL Population by SNP. 农业生物技术学报, 2019, 27(9): 1542-1549.

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http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.09.003 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I9/1542

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