铁皮石斛SPL基因家族鉴定及表达分析

doi:10.3969/j.issn.1674-7968.2025.05.007

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Abstract SQUAMOSA promoter binding protein-like (SPL) is a transcription factor that plays a crucial role in plant growth and development, as well as stress response. In this study, the DoSPL gene family was identified based on the whole genome of Dendrobium officinale through the bioinformatics approach. Their chromosome position, composition of promoter elements, gene structure, phylogeny, and subcellular localization were analyzed. Meanwhile, their expression patterns in different tissues and under stresses were detected using real-time quantitative reverse transcription PCR (qRT-PCR). Results showed that D. officinale harbored a total of 16 SPL family members, all contained SBP zinc finger structure, and divided into 7 subfamilies. Majority of them were hydrophilic proteins, located in the nucleus, anchored on 10 chromosomes, and possessed 3 tandem repeat gene pairs. Many regulatory elements associated with growth and development, and stress response were presented in the upstream promoter regions of DoSPLs. Multi-species phylogenetic analysis revealed that D. officinale was phylogenetically close to rice (Oryza sativa) SPL proteins. Gene expression analysis showed that DoSPLs were significantly and differentially expressed in different tissues of D. officinale, and exhibited high expression in flowers. Most DoSPLs genes were evidently upregulated or downregulated under the induction of low temperature, methyl jasmonate, or salt stress. Specially, DoSPL12 was significantly upregulated under the above stresses, and might play a crucial role in stress responses. This study provides fundamental data for further exploration the function of the DoSPL gene family.

Key words： Dendrobium officinale SQUAMOSA promoter binding protein-like (SPL) gene family Stress response Gene expression

Received: 10 July 2024

ZTFLH:

S682.31

Corresponding Authors: *yuzhenming@zcmu.edu.cn; shenxiaoxia@zcmu.edu.cn

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	Articles by authors
	ZHENG Fei-Xiong
	XU Zhang-Ting
	SUN Yi-Ming
	JIANG Lin-Qi
	YU Zhen-Ming
	SHEN Xiao-Xia

Cite this article:

ZHENG Fei-Xiong,XU Zhang-Ting,SUN Yi-Ming, et al. Identification and Expression Analysis of SPL Gene Family in Dendrobium officinale[J]. 农业生物技术学报, 2025, 33(5): 1018-1030.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.05.007 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I5/1018

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