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Cloning and Expression Analysis of the Zl-CRK5 Gene During the Swelling and Development of Stem in Zizania latifolia |
ZHENG Yu-Jie, ZHOU Ya-Ping, MA Ying-Ran, WANG Jing, TANG Jin-Tian, XIA Wen-Qiang, ZHANG Ya-Fen, YE Zi-Hong, CUI Hai-Feng* |
School of Life Sciences, China Jiliang University/Key Laboratory of Microbiological Metrology, Measurement & Bio-product Quality Security, State Administration for Market Regulation, Hangzhou 310018, China |
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Abstract The swollen stem was induced by the infection of Ustilago esculenta in Zizania latifolia, following with the inhibition of polar growth of plants and the down-regulation of expression of auxin (IAA) polar transport related genes. In this research, the calcium-dependent protein kinase (CDPK) related kinase 5 (CRK5) gene of Z. latifolia was cloned with the full-length gDNA sequence of 4 224 bp, encoding 453 amino acids and containing the typical STKc_CAMK conformal domain of CRK kinase family. It was closely related to Oryza brachyantha. qPCR analysis showed that Zl-CRK5 was highly expressed in stem and leaf sheath of Z. latifolia (P<0.05), but lower in root and leaf (P<0.05). The expression of Zl-CRK5 in stems was significantly down-regulated (P<0.05) after 7 d infection of U. esculenta in plantlets of Z. latifolia. It was significantly down-regulated during the initial swolling stem of normal Z. latifolia (P<0.05), and contained lower expression level following the subsequent swollen and development (P<0.05), which was similar to the reported gene of Zl-PIN1b in Z. latifolia. Further analysis showed that the Zl-CRK5 gene and IAA polar transport-related genes PIN (PIN-formed) of Zl-PIN1b and Zl-PIN1c showed a coordinated expression relationship in the stems of Z. latifolia after treatment with IAA polar transport-inhibitor of N-1-Naphthylphthalamic (NPA). It was speculated that Zl-CRK5 might be involved in the regulation of polar growth of stems by activating the expression of IAA polar transport-related genes Zl-PIN1b and Zl-PIN1c. Further research would help to reveal the regulatory mechanism of stem expansion induced by infection of U. esculenta.
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Received: 13 May 2024
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Corresponding Authors:
*hfcui@cjlu.edu.cn
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