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| Isolation, Identification and Control Effect of Bacillus velezensis ZF513 Against Damping-off of Cucumber (Cucumis sativus) |
| YAN Li-Xia*, LI Lei*, DING Chang-Zong, SHI Yan-Xia, CHAI A-Li, FAN Teng-Fei, XIE Xue-Wen**, LI Bao-Ju** |
| Institute of Vegetables and Flowers/State Key Laboratory of Vegetable Biobreeding, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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Abstract Damping-off of cucumber caused by Pythium aphanidermatum is a serious seedling disease in cucumber (Cucumis sativus) production, which seriously affects the yield and quality of cucumber. In order to obtain a biocontrol strain with antagonistic effect against damping-off of cucumber, in this study, Bacillus spp. were isolated from the inter-root soil of healthy cucumber using a gradient dilution plating method, and obtained the biocontrol strain ZF513 with the best preventive effect against damping-off of cucumber, which amounted to 86.64%, through potted plants in greenhouse. After morphological, physiological and biochemical tests, unique carbon source utilization determination and molecular identification, it was determined that the biocontrol strain ZF513 was Bacillus velezensis. Plate confrontation method was used to determine that strain ZF513 had obvious antagonistic effect on 8 kinds of plant pathogens (Pythium aphanidermatum, Rhizoctonia solani, Fusarium oxysporum, F. solani, Corynespora cassiicola, Phytophthora capsici, Botrytis cinerea, Mphylium solani) with a broad-spectrum bacterial inhibition ability, in which the inhibition of P. aphanidermatum reached 65.23%. The results of pot test showed that strain ZF513 had obvious growth-promoting effect on cucumber plants, and the highest prevention effect on damping-off of cucumber was 72.61% when the concentration was 1×108 cfu/mL and the dosage was 10 mL/plant, and the strain could stabilize and colonize the inter-root soil of cucumber with the concentration of 105 cfu/g, which could protect the cucumber plants from disease invasion during the period of seedlings. In conclusion, strain ZF513 has potential application value in the control of damping-off of cucumber.
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Received: 29 December 2023
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Corresponding Authors:
**libaoju@caas.cn;xiexuewen@caas.cn
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| About author:: *These authors contributed equally to this work |
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