Abstract The skeleton is an important part of the animal body, playing a vital role in providing biomechanical support, maintaining movement, hematopoiesis, and calcium homeostasis. The application of bulk RNA sequencing (bulk RNA-seq) technology in skeletal research has provided important references for revealing the development of bone cells, but it still has limitations in revealing the heterogeneity of bone cells. In recent years, the emerging single-cell RNA sequencing (scRNA-seq) technology has greatly improved the efficiency of cell capture and survival rate, allowing researchers to interpret biological information from the perspective of individual cells in a population of cells, providing a new research strategy for studying the heterogeneity of bone cells and the relationship between cell populations. This article reviewed the progress of single-cell RNA sequencing technology and its application in animal bone research, with the aim of providing reference for future studies.
LIU Zhe,TIAN Xue-Kai,HE Yu-Lin, et al. Single-cell RNA Sequencing Technology and Its Application in Animal Bone Research[J]. 农业生物技术学报, 2024, 32(5): 1150-1168.
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