|
|
|
| Identification and Analysis of MYB Transcription Factors Related to Salt Stress in Pyrus betulaefolia based on Transcriptome Information |
| LI Hui1,*, ZHANG Yu-Feng1,2, LI Xiao-Gang1, WANG Zhong-Hua1, CHANG You-Hong1, LIN Jing1 |
1 Institute of Pomology/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2 College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China |
|
|
|
|
Abstract Myeloblastosis (MYB) is one of the most common transcription factor families in plants, which widely takes part in various adversities responses including salt stress. So far, there is still no systematic comparative analysis on the expression pattern of MYB family among various ecotypes with different salt tolerance abilities from the same species. Based on the transcriptome sequencing (RNA-Seq) data from the salt-tolerant ecotype and common ecotype of Pyrus betulaefolia, the differentially expressed PbMYBs genes before and after salt stress were screened and annotated in this study. Then, their conserved domains were classified, subcellular localizations were predicted and the phylogenetic tree was constructed. TBtools was used to draw the heatmaps to analyze the differential expression pattern of PbMYBs genes in roots, stems and leaves from the salt-tolerant ecotype and common ecotype of P. betulaefolia. By using transcriptome analysis tools, 129 PbMYBs transcription factors were annotated and selected from the RNA-Seq data, which differentially expressed in 2 ecotypes of P. betulaefolia after salt stress. These genes can be divided into 3 categories (1R-MYB, R2R3-MYB and 3R-MYB) according to their structural characteristics.The number of PbMYBs genes distributed on chromosome 15 of P. betulaefolia was the largest. The phylogenetic trees of MYB families were constructed, which showed that the PbMYBs family of P. betulaefolia contained 3 major branches, and 23 evolutionary branches. The results of the subcellular localization demonstrated that 42 PbMYBs were located in the extracellular, and 87 PbMYBs were located in the nucleus. Analysis of the differential expression pattern of PbMYBs based on transcriptome data showed that PbMYBs genes in P. betulaefolia may be involved in the transcriptional regulation of different organs in response to salt stress. Additionally, the results of real-time quantitative PCR further verified that some PbMYBs transcription factors may play a role in stress regulation in the roots, stems or leaves of P. betulaefolia, respectively. During the process of salt stress, the transcriptional regulation of 129 PbMYBs members in response to stress were diversity in various organs. The results of real-time quantitative PCR further verified that different PbMYBs transcription factors were up-regulated or down-regulated to response to salt stress signals in the roots, stems or leaves of P. betulaefolia, respectively. The results provide the relative information for further research on gene structure and biological function of the MYB family in P. betulaefolia.
|
|
Received: 14 December 2022
|
|
|
|
Corresponding Authors:
* lihui7904@163.com
|
|
|
|
[1] 郭媛, 平文静, 黄亚群, 等. 2020. 植物中过表达MYB转录因子调控盐胁迫响应的整合分析[J]. 沈阳农业大学学报, 51(1): 35-42.
(Guo Y, Ping W J, Huang Y Q, et al.2020. Meta-analysis of the effects of overexpressing of MYB transcription factors on plant responses to salt stress[J]. Journal of Shenyang Agricultural University, 51(1): 35-42. )
[2] 李君霞, 王春义, 丁宇涛, 等. 2020. MYB转录因子在植物耐盐基因工程中的应用进展[J]. 浙江农业学报, 32(10):1910-1920.
(Li J X, Wang C Y, Ding Y T, et al.2020. Progress on application of MYB transcription factor in plant salt tolerance genetic engineering[J]. Acta Agriculturae Zhejiangensis, 32(10): 1910-1920.)
[3] 李星伟, 王庆江, 吴宇童, 等. 2021. 苹果MdMYB113基因响应高盐胁迫的功能鉴定[J]. 植物生理学报, 57(3): 579-588.
(Li X W, Wang Q J, Wu Y T, et al.2021. Functional identification of MdMYB113 gene in apple in response to high salt stress[J]. Plant Physiology Journal, 57(3): 579-588.)
[4] Abe H, Urao T, Ito T, et al.2003. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling[J]. The Plant Cell, 15(1):63-78.
[5] Cao Y P, Han Y H, Li D H, et al.2016. MYB transcription factors in Chinese pear (Pyrus bretschneideri Rehd.): Genome-wide identification, classification, and expression profiling during fruit development[J]. Frontiers in Plant Science, 7: 577.
[6] Cao Z H, Zhang S Z, Wang R K, et al.2013. Genome wide analysis of the apple MYB transcription factor family allows the identification of MdoMYB121 gene conferring abiotic stress tolerance in plant[J]. PLOS ONE, 8(7): e6995.
[7] Chen C J, Chen H, Zhang Y, et al.2020. TBtools: An integrative toolkit developed for interactive analyses of big biological data[J]. Molecular Plant, 13(8): 1194-1202.
[8] Crooks G E, Hon G, Chandonia J M, et al.2004. Web Logo: A sequence logo generator[J]. Genome Research, 14(6): 1188-1190.
[9] Dong W, Liu X J, Li D L, et al.2018. Transcriptional profiling reveals that a MYB transcription factor MsMYB4 contributes to the salinity stress response of alfalfa[J]. PLOS ONE, 13(9): e0204033.
[10] Dong X G, Wang Z, Tian L M, et al.2020. De novo assembly of a wild pear (Pyrus betulaefolia) genome[J]. Plant Biotechnology Journal, 18(2): 581-595.
[11] Du B Y, Liu H, Dong K T, et al.2022. Over-Expression of an R2R3 MYB gene, MdMYB108L, enhances tolerance to salt stress in transgenic plants[J]. International Journal of Molecular Sciences, 23: 9428.
[12] Dubos C, Stracke R, Grotewold E, et al.2010. MYB transcription factors in Arabidopsis[J]. Trends in Plant Science, 15(10): 573-581.
[13] Feng S Q, Xu Y C, Yang L, et al.2015. Genome-wide identification and characterization of R2R3-MYB transcription factors in pear[J]. Scientia Horticulturae, 197(17): 176-182.
[14] Kim J H, Nguyen N H, Jeong C Y, et al.2013. Loss of the R2R3 MYB, AtMyb73, causes hyper-induction of the SOS1 and SOS3 genes in response to high salinity in Arabidopsis[J]. Journal of Plant Physiology, 170(16): 1461-1465.
[15] Li H, Liu W, Yang Q S, et al.2018. Isolation and comparative analysis of two Na+/H+ antiporter NHX2 genes from Pyrus betulaefolia[J]. Plant Molecular Biology Reporter, 36(3): 439-450.
[16] Li H, Zhang Y F, Zhou X Y, et al.2022a. Single-base resolution methylome of different ecotype from Pyrus betulaefolia reveals epigenomic changes in response to salt stress[J]. Scientia Horticulturae, 306: 111437.
[17] Li J B, Zhou H, Xiong C W, et al.2022b. Genome-wide analysis R2R3-MYB transcription factors in Xanthoceras sorbifolium Bunge and functional analysis of XsMYB30 in drought and salt stresses tolerance[J]. Industrial Crops & Products, 178: 114597.
[18] Li X L, Xue C, Li J M, et al.2016. Genome-wide identification, evolution and functional divergence of MYB transcription factors in Chinese white pear (Pyrus bretschneideri)[J]. Plant and Cell Physiology, 57(4): 824-847.
[19] Rahaie M, Xue G P, Naghavi M R, et al.2010. A MYB gene from wheat (Triticum aestivum L.) is up-regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes[J]. Plant Cell Reports, 29(8): 835-844.
[20] Tang Y H, Bao X X, Zhi Y L, et al.2019. Over-expression of a MYB family gene, OsMYB6, increases drought and salinity stress tolerance in transgenic rice[J]. Frontiers in Plant Science, 10: 168.
[21] Wang R, Jing W, Xiao L Y, et al.2015. The rice high-affinity potassium transporter 1;1 is involved in salt tolerance and regulated by an MYB-type transcription factor[J]. Plant Physiology, 168(3): 1076-1090.
[22] Wang Y J, Zhang Y, Fan C J, et al.2021. Genome-wide analysis of MYB transcription factors and their responses to salt stress in Casuarina equisetifolia[J]. BMC Plant Biology, 21: 328.
[23] Wei Q H, Chen R, Wei X, et al.2020. Genome-wide identification of R2R3-MYB family in wheat and functional characteristics of the abiotic stress responsive gene TaMYB344[J]. BMC Genomics, 21: 792.
[24] Xu F C, Liu H L, Xu Y Y, et al.2018. Heterogeneous expression of the cotton R2R3-MYB transcription factor GbMYB60 increases salt sensitivity in transgenic Arabidopsis[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 133(1): 15-25.
[25] Zhang X, Chen L C, Shi Q H, et al.2020. SlMYB102, an R2R3-type MYB gene, confers salt tolerance in transgenic tomato[J]. Plant Science, 291: 110356.
[26] Zhang Z J, Zhang L, Liu Y, et al.2022. Identification and expression analysis of R2R3-MYB family genes associated with salt tolerance in Cyclocarya paliurus[J]. International Journal of Molecular Sciences, 23: 3429.
[27] Zhao Y Y, Yang Z E, Ding Y P, et al.2019. Over-expression of an R2R3 MYB gene, GhMYB73, increases tolerance to salt stress in transgenic Arabidopsis[J]. Plant Science, 286: 28-36. |
|
|
|
