土地类芽胞杆菌NK3-4对藤仓镰孢菌基因表达的影响

doi:10.3969/j.issn.1674-7968.2024.02.015

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Abstract Paenibacillus terrae NK3-4 strain is found to be antagonistic to plant pathogens. However, the response mechanism of NK3-4 interaction with the pathogen remains unclear. In this study, the inhibitory effect of NK3-4 on rice (Oryza sativa) bakanae disease pathogenic Fusarium fujikuroi was measured, and a transcriptome sequencing analysis for the NK3-4 inhibited F. fujikuroi was taken. The results showed that NK3-4 inhibited the growth, pigment synthesis and conidia formation of F. fujikuroi, and damaged the mycelia. Among the 2 291 differential expressed genes in F. fujikuri induced by NK3-4, the number of down-regulated genes was 2.44 times of up-regulated genes. And the down-regulated genes were mainly enriched in nucleus (GO:0005634), protein-containing complex (GO:0032991), intracellular non-membrane-bound organelles (GO:0043232) and other functional groups. Among the differential expressed genes, the number of down-regulated expressed genes related to pathogenesis (GO:0009405), toxin biosynthetic process (GO:0009403), aflatoxin biosynthetic process (GO:0045122), reproduction (GO:0000003 ) and other relative functions were more than that of the up-regulated genes. Meanwhile, of the differential expressed genes which involved in cell wall chitin biosynthetic process (GO:00060382), asexual reproduction (GO:0019954), conidia formation (GO:0048315), conidia development (GO:0061794), pigment biosynthetic process (GO:0046148) and other relative functions were all down-regulated expressed. The result of reverse transcription fluorescence quantitative PCR detection was consistent with transcriptome sequencing result (Pearson correlation index 0.90), indicating NK3-4 down-regulated the expression levels of genes in F. fujikuroi. Synthetic analysis showed that NK3-4 may inhibit F. fujikuroi by down-regulating the expression of genes, which related to growth and reproduction, pigment synthesis and spore production, and may reduce its virulence by inhibiting the biosynthesis of toxin in F. fujikuroi. The results provide a basis for further elucidating the molecular mechanism of NK3-4 interacting with F. fujikuroi.

Key words： Paenibacillus terrae Fusarium fujikuroi Transcriptome Antimicrobial mechanism

Received: 10 February 2023

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Corresponding Authors: *wenqingyu09@163.com; nkkxylp@163.com

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	LIU Wen-Zhi
	WU Xin-Yu
	DU Jia-Peng
	PENG Zhi-Hong
	GONG Sha-Sha
	YU Wen-Qing
	LI Peng

Cite this article:

LIU Wen-Zhi,WU Xin-Yu,DU Jia-Peng, et al. Effects of Paenibacillus terrae NK3-4 on Gene Expression in Fusarium fujikuroi[J]. 农业生物技术学报, 2024, 32(2): 427-443.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2024.02.015 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2024/V32/I2/427

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