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| Identification of the SNAC Gene Family in Eucalyptus grandis and Its Expression Analysis Under Abiotic Stresses |
| HONG Jia-Du, NI Xiao-Xiang, YU Jian-Feng, WU Meng-Jie, ZHAO Shuang, CHENG Long-Jun* |
| State Key Laboratory of Subtropical Silviculture/College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China |
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Abstract Eucalyptus grandis has made important contribution to wood-related industries in South China, but they are sensitive to abiotic stresses, limiting the expansion of its cultivation and benefits improvement of plantation. NAC is a plant-specific transcription factor family, which is widely involved in plant growth, development, metabolism and stress response. And, the stress-related NACs were named SNAC. In this study, 166 NAC transcription factors identified in E. grandis genome and 74 SNAC in other plant species were used to identify EgrSNACs. And, EgrSNACs structure, coding protein sequence, chromosome localization, collinearity and gene duplication, cis-acting elements on promoters, and the tissue-specific expression patterns were analyzed. In addition, the seedlings of E. grandis were treated with low temperature, drought, high salt, abscisic acid (ABA) and methyl jasmonate (MeJA), and qPCR was used to analyze the expression of EgrSNACs under these treatments. The results showed that there were 22 EgrSNACs in E. grandis, belonging to ATAF, NAP, and AtNAC3 subfamily. Except for EgrSNAC20, the proteins encoded by these genes all contained 5 subdomains of the typical NAM domain, corresponding to Motif1~Motif5. The 22 EgrSNACs were distributed on 9 chromosomes, with 4 collinear gene pairs and 2 tandem repeat gene segments composed of 11 EgrSNACs. Cis-acting element of promoter analysis showed that multiple stress response elements were distributed on the EgrSNAC promoters. The expression pattern of tandem duplicated EgrSNACs were similar in leaf, stem, xylem and phloem. The qPCR analysis of different time treatments at 4 ℃ indicated that the expression of the 19 EgrSNACs were induced by low temperature, in addition to EgrSNAC1, EgrSNAC3, and EgrSNAC22; there were 19 EgrSNACs that responded to the drought, except EgrSNAC2, EgrSNAC5, EgrSNAC14 and EgrSNAC21 were suppressed, others were induced. Under high salt treatment, 19 EgrSNACs expression changes, and only EgrSNAC21 was suppressed. In addition, 14 EgrSNACs were induced under ABA (100 μmol/L) treatment. 13 EgrSNACs were induced and 4 were inhibited under MeJA (100 μmol/L) treatment . The results of this study revealed the relationship between EgrSNACs and their response of low temperature, drought, high salt, ABA, MeJA and other abiotic stress factors, providing a reference for further study of EgrSNACs in E. grandis.
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Received: 26 December 2022
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Corresponding Authors:
* ljcheng@zju.edu.cn
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[1] Ahmad M, Yan X, Li J, et al.2018. Genome wide identification and predicted functional analyses of NAC transcription factors in Asian pears[J]. BMC Plant Biology, 18(1): 1-15.
[2] Alshareef N O, Wang J Y, Ali S, et al.2019. Overexpression of the NAC transcription factor JUNGBRUNNEN1 (JUB1) increases salinity tolerance in tomato[J]. Plant Physiology and Biochemistry, 140(7): 113-121.
[3] Blanc G, Wolfe K H.2004. Widespread paleopolyploidy in model plant species inferred from age distributions of duplicate genes[J]. Plant Cell, 16(7): 1667-1678.
[4] Chen X, Wang Y, Lv B, et al.2014. The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway[J]. Plant and Cell Physiology, 55(3): 604-619.
[5] Christianson J A, Dennis E S, Llewellyn D J, et al.2010. ATAF NAC transcription factors: Regulators of plant stress signaling[J]. Plant Signaling & Behavior, 5(4): 428-432.
[6] Deng R, Zhao H, Xiao Y, et al.2019. Cloning, characterization, and expression analysis of eight stress-related NAC genes in Tartary Buckwheat[J]. Crop Science, 59(1): 266-279.
[7] Fang Y, You J, Xie K, et al.2008. Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice[J]. Molecular Genetics and Genomics, 280(6): 547-563.
[8] Huang L, Hong Y, Zhang H, et al.2016. Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance[J]. BMC Plant Biology, 16(1): 1-18.
[9] Jeong J S, Kim Y S, Redillas M C, et al.2013. OsNAC5 overexpression enlarges root diameter in rice plants leading to enhanced drought tolerance and increased grain yield in the field[J]. Plant Biotechnology Journal, 11(1): 101-114.
[10] Jin H, Huang F, Cheng H, et al.2013. Overexpression of the GmNAC2 gene, an NAC transcription factor, reduces abiotic stress tolerance in tobacco[J]. Plant Molecular Biology Reporter, 31(2): 435-442.
[11] Li W, Zeng Y, Yin F, et al.2021. Genome-wide identification and comprehensive analysis of the NAC transcription factor family in sunflower during salt and drought stress[J]. Scientific Reports, 11(1): 1-12.
[12] Liang K H, Wang A B, Yuan Y H, et al.2020. Picea wilsonii NAC transcription factor PwNAC30 negatively regulates abiotic stress tolerance in transgenic Arabidopsis[J]. Plant Molecular Biology Reporter, 38(4): 554-571.
[13] Liu J H, Peng T, Dai W.2014. Critical cis-acting elements and interacting transcription factors: Key players associated with abiotic stress responses in plants[J]. Plant Molecular Biology Reporter, 32(2): 303-317.
[14] Lu X, Dun H, Lian C, et al.2017. The role of peu-miR164 and its target PeNAC genes in response to abiotic stress in Populus euphratica[J]. Plant Physiology and Biochemistry, 115: 418-438.
[15] Mantri N, Patade V, Penna S, et al.2012. Abiotic Stress Responses in Plants: Present and Future[C]. Abiotic Stress Responses in Plants. Springer, New York, NY, pp. 1-19.
[16] Mao X, Chen S, Li A, et al.2014. Novel NAC transcription factor TaNAC67 confers enhanced multi-abiotic stress tolerances in Arabidopsis[J]. PLOS ONE, 9(1): e84359.
[17] Marques D N, dos Reis S P, de Souza C R.2017. Plant NAC transcription factors responsive to abiotic stresses[J]. Plant Gene, 11: 170-179.
[18] Nakashima K, Takasaki H, Mizoi J, et al.2012. NAC transcription factors in plant abiotic stress responses[J]. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1819(2): 97-103.
[19] Nakashima K, Tran L S P, Van Nguyen D, et al.2007. Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice[J]. The Plant Journal, 51(4): 617-630.
[20] Nuruzzaman M, Manimekalai R, Sharoni A M, et al.2010. Genome-wide analysis of NAC transcription factor family in rice[J]. Gene, 465(1-2): 30-44.
[21] Nuruzzaman M, Sharoni A M, Kikuchi S.2013. Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants[J]. Frontiers in Microbiology, 4(9): 248.
[22] Olsen A N, Ernst H A, Leggio L L, et al.2005. NAC transcription factors: Structurally distinct, functionally diverse[J]. Trends in Plant Science, 10(2): 79-87.
[23] Ooka H, Satoh K, Doi K, et al. 2003. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana[J]. DNA Research, 10(6): 239-247.
[24] Saha D, Shaw A K, Datta S, et al.2021. Evolution and functional diversity of abiotic stress-responsive NAC transcription factor genes in Linum usitatissimum L[J]. Environmental and Experimental Botany, 188(8): 104512.
[25] Seok H Y, Woo D H, Nguyen L V, et al.2017. Arabidopsis AtNAP functions as a negative regulator via repression of AREB1 in salt stress response[J]. Planta, 245(2): 329-341.
[26] Takasaki H, Maruyama K, Kidokoro S, et al.2010. The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice[J]. Molecular Genetics and Genomics, 284(3): 173-183.
[27] Tanin M J, Saini D K, Sandhu K S, et al.2022. Consensus genomic regions associated with multiple abiotic stress tolerance in wheat and implications for wheat breeding[J]. Scientific Reports, 12(1): 1-17.
[28] Wang L, Hu W, Sun J, et al.2015. Genome-wide analysis of SnRK gene family in Brachypodium distachyon and functional characterization of BdSnRK2.9[J]. Plant Science, 237: 33-45.
[29] Wang X, Yao S, Htet W P P M, et al.2022. MicroRNA828 negatively regulates lignin biosynthesis in stem of Populus tomentosa through MYB targets[J]. Tree Physiology, 42(8): 1646-1661.
[30] Wu Y, Deng Z, Lai J, et al.2009. Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses[J]. Cell Research, 19(11): 1279-1290.
[31] Yarra R, Wei W.2021. The NAC-type transcription factor GmNAC20 improves cold, salinity tolerance, and lateral root formation in transgenic rice plants[J]. Functional & Integrative Genomics, 21(3): 473-487.
[32] Zhang H, Ma F, Wang X, et al.2020. Molecular and functional characterization of CaNAC035, an NAC transcription factor from pepper (Capsicum annuum L.)[J]. Frontiers in Plant Science, 11(4): 14.
[33] Zhang H, Zhu J, Gong Z, et al.2022. Abiotic stress responses in plants[J]. Nature Reviews Genetics, 23(2): 104-119.
[34] Zhu T, Nevo E, Sun D, et al.2012. Phylogenetic analyses unravel the evolutionary history of NAC proteins in plants[J]. Evolution: International Journal of Organic Evolution, 66(6): 1833-1848. |
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