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| Identification of the CBF Gene Family in Phaseolus vulgaris and Its Expression Analysis Under Low Temperature Stress |
| LONG Zi-Xuan, LIU Wei, WANG Yu-Ping*, ZHANG Xiao-Xu, LUO Xin-Rui, WANG Zhi |
| College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China |
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Abstract CBF (C-repeat binding factor) is a key transcription factor that can be rapidly induced and plays an important role in the response of plants to chilling stress. In this study, bioinformatics analysis of the common bean (Phaseolus vulgaris) CBF gene family and the expression of common bean CBF genes under chilling stress were examined using transcriptome data (RNA-seq) and qRT-PCR. The results showed that a total of 7 PvCBFs were identified in common bean, unevenly distributed on 5 chromosomes; phylogenetic analysis revealed that common bean CBF members were classified into 4 subgroups (Ⅰ~Ⅳ), which were identical to the results of gene structure and conserved motif analysis; covariance analysis revealed that there were 5 pairs of CBF gene families between common bean and Arabidopsis thaliana. The promoter analysis revealed that PvCBFs contained a variety of cis-elements involved in plant growth and development, hormone and stress response. The qRT-PCR results showed that 3 PvCBFs were significantly up-regulated under chilling stress (P<0.05). This study can provide a reference for subsequent study of the chilling tolerance function of PvCBF genes.
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Received: 08 August 2022
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Corresponding Authors:
*wangyp@gsau.edu.cn
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