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| Effect of Different Rootstocks on Fruit Quality and Expression of Genes Related to Gluconeogenesis in 'Shine Muscat' Grapes (Vitis vinfera) |
| SHEN Le-Yi1, WANG Li-Ru2, XU Yue1,3, CHEN Tian-Chi1, XU Tao1, GUO Yan-Fei1, FANG Cong-Ling2, FAN Lin-Jie2, WU Yue-Yan1,* |
1 College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315000, China;
2 Cixi City Forestry Technology Extension Center, Ningbo 315000, China;
3 Institute of Virus and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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Abstract Grapes (Vitis vinfera) are one of the four major fruits in the world, and their excellent cultivation depends to a large extent on grafting, which not only improves the resistance of the fruit trees, but also influences the fruit quality. The objective of this study was to investigate the effects of different rootstocks on the fruit quality and the expression of gluconeogenesis related genes in 'Shine Muscat' grapes, and to screen out rootstock combinations with excellent overall quality in Jiangsu and Zhejiang regions. In this study, samples of 'Shine Muscat' grapes grafted on 12 rootstocks were used to determine various quality indicators and to analyze the expression of phosphoenolpyruvate carboxykinase (PEPCK), pyruvate phosphate dikinase (PPDK), fructose-1,6-bisphosphatase (FBP) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes by real-time fluorescence quantitative PCR. The results showed that grafting of 12 rootstocks affected the quality index of 'Shine Muscat' grapes to varying degrees, especially the content of amino acids and sugar-acid fractions. Among the 12 rootstock combinations, '8B' rootstock grafted on 'Shine Muscat' gave the largest fruit size and hardness, '1103P' and '5BB' rootstocks grafted on 'Shine Muscat' gave the largest fruit solid-acid ratio, and 'Beda' rootstock grafted on 'Shine Muscat' gave the highest content of various amino acids in the fruit; '101-14' and '5BB' rootstocks had the highest glucose and fructose contents in the fruit after grafting 'Shine Muscat', while '3309C' rootstock grafted 'Shine Muscat' had the lower glucose and fructose contents in the fruit among the 12 rootstock combinations, but the content of acids such as tartaric acid, malic acid and citric acid was higher. Rootstock grafting had an effect on the expression of sugar anabolism-related genes, but it had the greatest effect on the expression of PEPCK gene, and the expression levels of PEPCK, PPDK, FBP and GAPDH were the highest in fruits of 'Shine Muscat' grafted on '101-14' rootstock. The expression of PEPCK genes in different rootstock combinations was significantly and positively correlated with glucose and fructose content, and it was hypothesized that different rootstocks affect the regulation of PEPCK gene in grape flesh, thus affecting conversion of organic acids to sugars and eventually the sugar accumulation. The top 3 rootstock combinations ranked in order by principal component analysis were 8B/SM, Beda/SM, and K3/SM. This study provides a theoretical basis for quality rootstock selection.
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Received: 08 December 2022
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Corresponding Authors:
*wyynb2009@163.com
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