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| Effect of Overexpression of F-box Gene BoFBX117 on Low-temperature Tolerance of Cabbage (Brassica oleracea var. capitata) |
| LUO Yu-Xia, XU Tao, DAI Qian, CHEN Yao, SONG Jiang-Hua* |
| School of Horticulture, Anhui Agricultural University, Hefei 230036, China |
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Abstract F-box gene plays an important role in plant abiotic stress response. In order to study the function of F-box gene BoFBX117 (GenBank No. XM_013731217.1) in cabbage (Brassica oleracea var. capitata) response to low-temperature stress, in this study, the BoFBX117 over-expression vector was constructed, and the over-expression of BoFBX117 cabbage (OE-BoFBX117) plants were obtained by Agrobacterium tumefaciens mediated method. After 24 h of low-temperature stress at 4, 0, -2 and -4 ℃, the phenotype of the over-expression of BoFBX117 cabbage showed that the degree of leaf yellowing wilting was less than that of non-transgenic cabbage (WT). After 7 d of recovery, it was found that the over-expression of BoFBX117 cabbage had stronger ability to return to normal growth. The results of qPCR showed that the expression of BoFBX117 in transgenic plants was significantly increased under low-temperature stress (P<0.01), which was 1.87~4.80 times that of WT plants. Under low-temperature stress at 0 ℃, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of transgenic plants were the highest, which were 80%, 16% and 99% higher than those of WT plants respectively. Under low-temperature stress, the content of malondialdehyde (MDA) continuously accumulated. At -4 ℃, the MDA content of transgenic plants was 0.62 μmol/g FW, 53.38% lower than WT. Under normal conditions and low-temperature stress, the proline and soluble sugar contents of transgenic plants were significantly higher than those of WT plants (P<0.05). The results showed that BoFBX117 might play a positive role in the response to low-temperature stress, and over-expression of BoFBX117 in cabbage could improve the low-temperature tolerance of cabbage. This study provides support for the breeding of low-temperature tolerance varieties of cabbage.
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Received: 15 December 2022
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Corresponding Authors:
* jhsong@ahau.edu.cn
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