早孕因子(EPF)在雌性牦牛主要生殖器官中的表达

doi:10.3969/j.issn.1674-7968.2020.05.011

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Abstract Early pregnancy factor (EPF), a kind of pregnancy related protein, plays an important role in the development of embryo as an autocrine and paracrine immune tolerance factor and growth factor of trophoblast layer. In this study, ovary, uterus, fallopian tube and placenta samples were collected respectively from female yak (Bos grunniens) during follicular phase, luteal phase and early pregnant period to investigate the expression of EPF and it role in reproduction. qRT-PCR, Western blott and immunohistochemistry (IHC) techniques were used to detect the expression of EFP mRNA and protein. The result indicated that EPF protein was most distributed in uterine glandular epithelial cells, endometrial epithelial cells, oviduct epithelial cells, membranous luteal cells, placental trophoblastic giant cells and crypt epithelial cells. qRT-PCR results showed that expression level of EPF mRNA in the ovary was significantly lower during follicular phase than that during early pregnant period and luteal phase. In oviduct and uterus the expression level of EPF mRNA was significantly higher during the follicular phase than that during the luteal phase and early pregnant period. The expression of EPF mRNA in ovarian and maternal placenta during early pregnant period was significantly higher than other reproductive organs. Western blot results showed that the expression of EPF protein in ovarian and oviduct during the early pregnant period was significantly higher than that during the luteal phase and follicular phase. In uterus of the follicular phase, the expression of EPF protein was significantly higher than that during the early pregnant period and luteal phase. It was noticeable that the expression of EPF in ovary and maternal placenta in early pregnant period was significantly higher than other reproductive organs, especially in the ovary. The results showed that EPF was spatiotemporal differently expressed in the main reproductive organs of female yak. During early pregnancy, EPF was mainly derived from ovary and maternal placenta. This study provides some useful materials to investigate the whole role of EPF in reproduction of yak.

Key words： Early pregnant factor (EPF) Yak Western blot qRT-PCR

Received: 10 October 2019

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S857.2+1

Corresponding Authors: *fanjf@gsau.edu.cn

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	ZHAO Sheng-Xian
	FAN Jiang-Feng
	YU Si-Jiu
	CUI Yan
	HAN Xiao-Hong
	MA Yue
	HE Hong-Hong
	MA Jin-Biao
	CAO Yan-Tao
	Bao Mei

Cite this article:

ZHAO Sheng-Xian,FAN Jiang-Feng,YU Si-Jiu, et al. Expression of Early Pregnancy Factors (EPF) in the Main Reproductive Organs of Female Yak (Bos grunnien)[J]. 农业生物技术学报, 2020, 28(5): 876-883.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.05.011 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I5/876

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