Abstract The regulation of angiogenic factors in the ovary of sheep (Ovis aries) is closely related to vascular proliferation, follicular development and corpus luteum formation, and has an important regulatory effect on the reproductive performance of sheep. In this study, qRT-PCR was used to detect the expression of VEGF gene in different ovarian ovary, and the expression and distribution of VEGF protein in ovarian tissue was analyzed by Immunohistochemistry and Western blot combined with IPP(image pro plus) technology. The results showed that VEGF was expressed in sheep ovary, and the expression of high fertility sheep was significantly higher than that of low fertility sheep (P<0.05). Studies showed that VEGF-positive signals were mainly localized in granulosa cells, luteal cells and vascular endothelial cells in different follicles of sheep, and were also weakly expressed in ovarian stroma and atresia follicular granulosa cells, the integrated optical density analysis of VEGF found that The expression of VEGF in the ovarian tissues of Small-tailed Han sheep, Hu sheep and Tibetan sheep was consistent. During the development of different follicles, the integrated optical density values of the follicles gradually increased and synchronized with follicular development. The positive signal strength was consistent with the follicle size, and the expression in the early stage of luteal formation was significantly higher than that in the degenerative stage. Researches found that high expression of VEGF could induce more angiogenesis around the follicles and corpus luteum in sheep, which improved the permeability of microvessels and capillaries and played an active role in increasing the number and quality of dominant follicles. This study provides basic information for revealing some of the genetic mechanisms of differences in sheep fecundity.
GUO Ya-Jun,WANG Xin-Rong. Distribution and Expression of VEGF in Ovarian Tissues of Sheep (Ovis aries) with Different Fertility[J]. 农业生物技术学报, 2020, 28(5): 866-875.
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