<em>LHR</em>、<em>PRLR</em>基因在不同发育阶段牦牛睾丸中的表达与定位

doi:10.3969/j.issn.1674-7968.2020.04.009

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Abstract Luteinizing hormone receptor (LHR) and prolactin receptor (PRLR) genes play essential roles in mammalian reproductive development. But still no any available research regarding these genes in yak (Bos grunniens). Therefore this study was planned to explore the role of LHR and PRLR genes in different tissues of Yak during different developmental ages (6, 24, 36 and 72 months). The mRNA expression levels of LHR and PRLR in the testis tissues of yak at different developmental stages and organs of yak were detected by qRT-PCR, while the protein expression of LHR and PRLR were evaluated by immunohistochemistry.The results showed that mRNA expression levels of LHR and PRLR genes in yak testis increased significantly from infancy to sexual maturity (P<0.01), but there was no significant difference between adult and sexual maturity. The expression level of LHR gene mRNA in different organs of adult yak was higher in testis than in kidney, skeletal muscle and lung (P<0.05). While the expression level of PRLR mRNA was higher in kidney, followed by skeletal muscle, testis and spleen (P<0.05). Additionally, immunohistochemistry findings revealed that LHR and PRLR proteins were located in testicular supporting cells, mesenchymal cells, primary spermatocytes and secondary spermatocytes, and they were increased gradually from infancy to sexual maturity stage and tended to be stable in adulthood. These findings displayed that LHR and PRLR may be vital for sperm maturation, in the biological functions of interstitial cells, and in regulating the development of other non-gonadal tissues. These results provide an important theoretical basis for revealing the molecular mechanism of testis development and reproductive regulation in yaks.

Key words： Yak Testis Luteinizing hormone receptor Prolactin receptor Gene expression Protein localization

Received: 14 September 2019

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Corresponding Authors: * pingyanlz@163.com

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	Articles by authors
	MA Fu-Long
	CHU Min
	CHANG Yong-Fang
	BAO Peng-Jia
	GUO Xian
	Qudratullah Kalwar
	MA Qi-Cai
	PAN He-Ping
	YAN Ping

Cite this article:

MA Fu-Long,CHU Min,CHANG Yong-Fang, et al. Expression and Localization of LHR and PRLR Genes in Testicles of Yak (Bos grunniens) at Different Developmental Stages[J]. 农业生物技术学报, 2020, 28(4): 672-680.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.04.009 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I4/672

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