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| Development and Application of 50K Liquid Phase Breeding Chip for Sheep(Ovis aries) |
| XU Ya-Nan1, CAO Li-Li2, SONG Li-Shuang1, LIU Xue-Fei1, LEI Jia-Ru1, JIANG Xi-Qing1, LI Guang-Peng1,*, YANG Lei1,* |
1 State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010070, China;
2 Department of Agriculture and Animal Husbandry of Inner Mongolia Autonomous Region, Hohhot 010070, China |
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Abstract With the advancement of molecular breeding technologies, genomic selection based on SNP markers has become a key technical approach for improving the efficiency of genetic improvement. Sheep(Ovis aries) are livestock of significant economic value, and developing efficient breeding tools tailored to their populations is crucial to advancing the sheep industry. This study developed a 50K SNP liquid phase breeding chip for sheep breeding using genomic data and targeted capture sequencing technology. By integrating SNP data from 355 individuals in the iSheep database and resequencing data from 10 East Friesian sheep, 58 859 high-quality SNP loci were selected, including 31 729 core functional loci(53.91%). The average spacing between the selected SNPs is 43.9 kb, covering functional regions associated with important economic traits. Quality testing of the chip was conducted by randomly sampling 100 sheep, and the results demonstrated excellent performance in the sheep population: The map rate exceeded 99.7%, capture rate was higher than 76%, dup rate was below 12%, and the SNP call rate reached over 95.98%. Further genome-wide association analysis used this breeding chip to identify genetic loci influencing sheep growth traits(such as body weight, body length, abdominal girth, and chest girth). The results showed that the chip successfully identified multiple significant SNP loci associated with growth traits. This chip holds broad application prospects in sheep molecular breeding, particularly in breeding efforts related to important economic traits, as it can help improve breeding efficiency and advance sheep genetic improvement. This study provides a scientific basis for future molecular marker selection of sheep growth traits
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Received: 01 December 2025
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Corresponding Authors:
*gpengli@imu.edu.cn; mrknowall@126.com
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