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| Fine Mapping of a QTL qFLW6 Controlling Rice (Oryza sativa) Flag Leaf Width |
| YU Jin-Sheng1,*, LU Hong-Yu1,*, LI Bai3, XING Yuan-Hang1, JIANG Wen-Jing1, ZHU Xin-Feng1, LUO Qiu-Hong1, NI Shen2# |
1 College of Advanced Agricultural Sciences, Zhejiang A&F University, Lin'an 311300, China;
2 State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Fuyang 311400, China;
3 Jiaxing Academy of Agricultural Sciences, Jiaxing 314001, China |
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Abstract Flag leaf width affects rice (Oryza sativa) plant type and photosynthetic efficiency, and is one of the important targets in rice breeding. In the chromosome segment substitution lines constructed by using 'Kolayal' and 'Huazhan' as donor and recurrent parents, it was found that the flag leaves of one certain line were significantly narrower than both parental lines, therefore named after 'narrow Huazhan'. The phenotype of the F2 segregation population of this line was observed, and the region of the QTL for the flag leaf width was located by map-based cloning and the relative expression of the candidate gene was analyzed. The array analysis of individuals with extreme phenotypes in the F2 segregation population showed that the QTL for flag leaf width was located on chromosome 6. Through fine mapping and comparative sequencing of critical recombinant individuals, quantitative locus for flag leaf width on chromosome 6 (qFLW6) was finally located in the 1.486 kb interval. As the candidate gene, Os06g0647200 had 2 alternative splicing forms, among which Os06g0647200-2 had a significantly higher expression level in the 'narrow Huazhan' than that in 'Huazhan'. This study identified a novel gene Os06g0647200 involved in regulating flag leaf width in rice, which provides valuable genetic resources for the improvement of flag leaf size and photosynthetic yield in rice.
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Received: 16 January 2026
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Corresponding Authors:
#jinshyu@zafu.edu.cn; nishen@caas.cn
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| About author:: * These authors contributed equally to this work |
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