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| OsAOC Participates in Rice (Oryza sativa) Resistance to Rice Blast and Bacterial Blight by Regulating Basal Immunity |
| LIN Qiao-Xia1, XU Dan-Jie1, LING Yu1, LI Zheng-Kang1, ZHUANG Xiao-Qian1, YI Zi-Jun1, LI Rui-Xue1, CHEN Xiao-Ting1,*, LU Guo-Dong2 |
1 College of Life Sciences/Fujian Provincial Key Laboratory for Plant and Microbe Interaction, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2 Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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Abstract Rice (Oryza sativa) allene oxide cyclase (OsAOC) is an important enzyme in the jasmonic acid (JA) biosynthesis pathway and is involved in development and stress responses. However, the research on the molecular mechanism of OsAOC in rice's disease resistance to rice blast and bacterial blight is rather limited. In this study, the expression levels of OsAOC in wild type 'Nipponbare' (NPB) were induced by the inoculation of Magnaporthe oryzae, methyl jasmonate (MeJA) and salicylic acid (SA) treatments. Then, the OsAOC knockout (OsAOC-KO) and OsAOC overexpression (OsAOC-OE) transgenic rice lines were obtained. Further study showed that OsAOC knockout led to growth defects and even death in rice. This phenotype could be alleviated by spraying MeJA to the seedlings. The plants overexpressing OsAOC showed enhanced resistance to the inoculation of M. oryzae and Xanthomonas oryzae pv. oryzae (Xoo). Under the induction of chitin and bacterial flagellin (flg22), reactive oxygen species (ROS) accumulation in OsAOC-OE was significantly higher than that of NPB (P<0.05). The expression of JA pathway genes (OsAOC and OsJAZ8) and SA pathway genes (OsPR1b and OsPAL1) in OsAOC-OE were up-regulated compared with NPB after inoculation with M. oryzae. Subcellular localization analysis showed that OsAOC protein was localized in chloroplasts. The interactions between OsAOC and OsOPR3 as well as OsbZIP81 were confirmed by yeast two-hybrid, glutathione-S-transferase (GST) pull-down and luciferase complementation assays. This study confirmed the localization and protein interaction of OsAOC in rice. OsAOC was involved in the pattern-triggered immunity (PTI) response of rice and mediated disease resistance response through the JA and SA pathways, positively regulating rice resistance to rice blast and bacterial blight. This study provides genetic resources for the breeding of disease-resistant rice varieties.
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Received: 12 January 2026
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Corresponding Authors:
*xiaotingchen@fafu.edu.cn
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[1] 周永林, 申小磊, 周立帅, 等. 2022. OsLOX10正调控水稻对稻瘟病和白叶枯病的抗性[J]. 中国水稻科学, 36(4): 348-356.
(Zhou Y L, Shen X L, Zhou L S, et al.2022. OsLOX10 positively regulates defense responses of rice to rice blast and bacterial blight[J]. Chinese Journal of Rice Science, 36(4): 348-356.)
[2] Agrawal G K, Jwa N S, Agrawal S K, et al.2003. Cloning of novel rice allene oxide cyclase (OsAOC): mRNA expression and comparative analysis with allene oxide synthase (OsAOS) gene provides insight into the transcriptional regulation of octadecanoid pathway biosynthetic genes in rice[J]. Plant Science, 164(6): 979-992.
[3] Chehab E W, Kim S, Savchenko T, et al.2011. Intronic T-DNA insertion renders Arabidopsis opr3 a conditional jasmonic acid-producing mutant[J]. Plant Physiology, 156(2): 770-778.
[4] Chen S, Songkumarn P, Liu J, et al.2009. A versatile zero background T-vector system for gene cloning and functional genomics[J]. Plant Physiology, 150(3): 1111-1121.
[5] Chen Z, Zheng W, Chen L, et al.2019. Green fluorescent protein- and Discosoma sp. red fluorescent protein-tagged organelle marker lines for protein subcellular localization in rice[J]. Frontiers in Plant Science, 10: 1421.
[6] Dean R, Kan J A L V, Pretorius Z A, et al.2012. The top 10 fungal pathogens in molecular plant pathology[J]. Molecular Plant Pathology, 13(4): 414-430.
[7] Devoto A, Ellis C, Magusin A, et al.2005. Expression profiling reveals COI1 to be a key regulator of genes involved in wound and methyl jasmonate-induced secondary metabolism, defense, and hormone interactions[J]. Plant Molecular Biology, 58(0): 497-513.
[8] Dubouzet J G, Maeda S, Sugano S, et al.2011. Screening for resistance against Pseudomonas syringae in rice-FOX Arabidopsis lines identified a putative receptor-like cytoplasmic kinase gene that confers resistance to major bacterial and fungal pathogens in Arabidopsis and rice[J]. Plant Biotechnology Journal, 9(4): 466-485.
[9] Gu Y H, Li G N, Wang P, et al.2022. A simple and precise method (Y2H-in-frame-seq) improves yeast two-hybrid screening with cDNA libraries[J]. Journal of Genetics Genomics, 49(6): 595-598.
[10] Guo H M, Li H C, Zhou S R, et al.2014. Cis-12-oxo-phytodienoic acid stimulates rice defense response to a piercing-sucking insect[J]. Molecular Plant, 7(11): 1683-1692.
[11] Guo J, Wu Y, Huang J, et al.2024. The Magnaporthe oryzae effector Avr-PikD suppresses rice immunity by inhibiting an LSD1-like transcriptional activator[J]. The Crop Journal, 12(2): 482-492.
[12] Harkenrider M, Sharma R, De Vleesschauwer D, et al.2016. Overexpression of rice wall-associated kinase 25 (OsWAK25) alters resistance to bacterial and fungal pathogens[J]. PLOS ONE, 11(0): e0147310.
[13] Hause B, Stenzel I, Miersch O, et al.2000. Tissue-specific oxylipin signature of tomato flowers: Allene oxide cyclase is highly expressed in distinct flower organs and vascular bundles[J]. The Plant Journal, 24(1): 113-126.
[14] Hazman M, Hause B, Eiche E, et al.2015. Increased tolerance to salt stress in OPDA-deficient rice ALLENE OXIDE CYCLASE mutants is linked to an increased ROS-scavenging activity[J]. Journal of Experimental Botany, 66(11): 3339-3352.
[15] Hong Y, Liu Q, Cao Y, et al.2019. The OsMPK15 negatively regulates Magnaporthe oryzae and Xoo disease resistance via SA and JA signaling pathway in rice[J]. Frontiers in Plant Science, 10(0): 752.
[16] Hu B, Zhou Y, Zhou Z, et al.2021. Repressed OsMESL expression triggers reactive oxygen species-mediated broad-spectrum disease resistance in rice[J]. Plant Biotechnology Journal, 19(7): 1511-1522.
[17] Kessler A, Halitschke R, Baldwin L T.2004. Silencing the jasmonate cascade: Induced plant defenses and insect populations[J]. Science, 305(5682): 665-668.
[18] Khush G S.2005. What it will take to feed 5.0 Billion Rice consumers in 2030[J]. Plant Molecular Biology, 59(1): 1-6.
[19] Liu D, Shi S, Hao Z, et al.2019. OsbZIP81, a homologue of Arabidopsis VIP1, may positively regulate JA levels by directly targeting the genes in JA signaling and metabolism pathway in rice[J]. International Journal of Molecular Sciences, 20(9): 2360.
[20] Liu W, Wang G L.2016. Plant innate immunity in rice: A defense against pathogen infection[J]. National Science Review, 3: 295-308.
[21] Ma X, Zhang Q, Zhu Q, et al.2015. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants[J]. Molecular Plant, 8(8): 1274-1284.
[22] Ngou B P M, Ahn H K, Ding P, et al.2021. Mutual potentiation of plant immunity by cell-surface and intracellular receptors[J]. Nature, 592(7852): 110-115.
[23] Peng X, Hu Y, Tang X, et al.2012. Constitutive expression of rice WRKY30 gene increases the endogenous jasmonic acid accumulation, PR gene expression and resistance to fungal pathogens in rice[J]. Planta, 236(5): 1485-1498.
[24] Qiu D, Xiao J, Ding X, et al.2007. OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate- and jasmonate-dependent signaling[J]. Molecular Plant-Microbe Interactions, 20(5): 492-499.
[25] Qiu D, Xiao J, Xie W, et al.2008. Rice gene network inferred from expression profiling of plants overexpressing OsWRKY13, a positive regulator of disease resistance[J]. Molecular Plant, 1(3): 538-551.
[26] Riemann M, Haga K, Shimizu T, et al.2013. Identification of rice allene oxide cyclase mutants and the function of jasmonate for defence against Magnaporthe oryzae[J]. The Plant Journal, 74(2): 226-238.
[27] Riemann M, Müller A, Korte A, et al.2003. Impaired induction of the jasmonate pathway in the rice mutant hebiba[J]. Plant Physiology, 133(4): 1820-1830.
[28] Roychowdhury R, Hada A, Biswas S, et al.2025. Jasmonic acid (JA) in plant immune response: Unravelling complex molecular mechanisms and networking of defence signaling against pathogens[J]. Journal of Plant Growth Regulation, 44(1): 89-114.
[29] Scalschi L, Sanmartín M, Camañes G, et al.2015. Silencing of OPR3 in tomato reveals the role of OPDA in callose deposition during the activation of defense responses against Botrytis cinerea[J]. The Plant Journal, 81(2): 304-315.
[30] Snoeren T A L, Broekgaarden C, Dicke M.2011. Jasmonates differentially affect interconnected signal-transduction pathways of Pieris rapae-induced defenses in Arabidopsis thaliana[J]. Insect Science, 18(3): 249-258.
[31] Sun T, Cen G, You C, et al.2020. ScAOC1, an allene oxide cyclase gene, confers defense response to biotic and abiotic stresses in sugarcane[J]. Plant Cell Reports, 39(12): 1785-1801.
[32] Taheri P, Tarighi S.2010. Riboflavin induces resistance in rice against Rhizoctonia solani via jasmonate-mediated priming of phenylpropanoid pathway[J]. Journal of Plant Physiology, 167(3): 201-208.
[33] Tao Z, Liu H, Qiu D, et al.2009. A pair of allelic WRKY genes play opposite roles in rice-bacteria interactions[J]. Plant Physiology, 151(2): 936-948.
[34] Tonnessen B W, Manosalva P, Lang J M, et al.2015. Rice phenylalanine ammonialyase gene OsPAL4 is associated with broad spectrum disease resistance[J]. Plant Molecular Biology, 87(3): 273-286.
[35] Yang L, Sun Q, Geng B, et al.2023. Jasmonate biosynthesis enzyme allene oxide cyclase 2 mediates cold tolerance and pathogen resistance[J]. Plant Physiology, 193(2): 1621-1634.
[36] Yuan M, Jiang Z, Bi G, et al.2021. Pattern recognition receptors are required for NLR-mediated plant immunity[J]. Nature, 592(7852): 105-109.
[37] Zhang F, Fang H, Wang M, et al.2022. APIP5 functions as a transcription factor and an RNA-binding protein to modulate cell death and immunity in rice[J]. Nucleic Acids Research, 50(9): 5064-5079.
[38] Zhao X, Zhang T, Feng H, et al.2021. OsNBL1, a multi-organelle localized protein, plays essential roles in rice senescence, disease resistance, and salt tolerance[J]. Rice, 14: 1-17. |
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