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| Identification of the SHR Subfamily Members of the Camellia sinensis GRAS Transcription Factors and Their Expression Analysis Under Abiotic Stress |
| SHEN Xiao-Hua, XIE Si-Yi, RAO Jia-Yi, DOU Xiang-Ya, WANG Jin-Bo, LIU Shuo-Qian* |
| College of Horticulture/Key Laboratory of Tea Science Ministry of Education, Hunan Agricultural University, Changsha 410128, China |
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Abstract As an important economic crop, the yield and quality of Camellia sinensis susceptible to environmental stresses, SHR subfamily transcription factors play key roles in plant development and stress response, but their functions in C. sinensis are still poorly studied. In order to analyse the regulatory mechanisms of SHR subfamily transcription factors in C. sinensis growth and development and abiotic stress response, this study used bioinformatics to analyse the physicochemical properties, phylogenetic relationships, protein structures, promoter cis-acting elements and chromosomal positioning of CsSHR subfamily members. Tissue-specific expression of the genes and their expression patterns under shade, drought, salt stress, methyl jasmonate (MeJA) and exogenous hormone (gibberellin A3 (GA3); 6-benzylaminopurine (6-BA)) treatments were further analysed by combining transcriptome data with qRT-PCR. The results showed that a total of 8 CsSHR genes were identified, and the encoded proteins were all unstable hydrophilic nuclear localised proteins, distributed on 6 chromosomes and with promoter regions enriched in light-responsive, hormone- and stress-related regulatory elements. Phylogenetic analyses showed that the subfamily of CsSHR was closely related to the SHR subfamily of Populus, and the gene structure showed that the small number of introns. Expression analysis showed that the members of the CsSHR gene family showed a tissue-specific expression pattern, in which CsSHR-1/4/7/8 was highly expressed in roots, while CsSHR-2/3/5 was predominantly expressed in young leaves. In response to abiotic stress, CsSHR-2 and CsSHR-5 were significantly up-regulated under drought and salt stress. Hormone treatment experiments showed that GA3 treatment induced the up-regulation of CsSHR-1~CsSHR-4 in the early stage, but inhibited the expression of CsSHR-5~CsSHR-8. However, 6-BA treatment led to a biphasic expression pattern in which most members were first inhibited and then up-regulated. The study showed that members of the CsSHR subfamily play important roles in the growth and environmental adaptation of C. sinensis through the regulation of tissue development and stress response. This study provides a new theoretical perspective for the study of the molecular breeding of C. sinensis stress tolerance.
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Received: 14 January 2025
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Corresponding Authors:
*shqianliu@sina.com
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