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| SVP Gene Cloning and Its Response to Temperature and Drought Stress in Green Onions (Allium fistulosum) |
| LIU Min1,3,*, KANG Yi-Feng2,*, ZHANG Yu-Chen1, WANG Yong-Qin1,** |
1 Vegetable Institute/National Key Laboratory of Vegetable Biological Breeding/National Engineering Technology Research Center/Beijing Key Laboratory of Vegetable Germplasm Improvement/North China Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
2 Beijing No.3 Middle School, Beijing 100035, China;
3 School of Plant Science, Xizang Agriculture and Animal Husbandry College, Nyingchi 850400, China |
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Abstract SVP (short vegetative phase) is one of the important genes in the regulation of plant flowering. In this study, the AfSVP gene (GenBank No. AfisC4G02946) of green onion (Allium fistulosum) was cloned. Its CDS was 744 bp and encoded for 247 amino acids. Bioinformatics analysis revealed that the AfSVP protein had a molecular weight of 60.35 kD and was a hydrophobic protein. Sequence alignment across multiple species demonstrated it was high degree of conservation; Following the introduction of the AfSVP gene into Nicotiana benthamiana protoplasts, laser-confocal microscopy was employed to visualize the protein's subcellular localization. The results showed that AfSVP was present in both the cytoplasm and the nucleus. Tissue-specific expression analysis further indicated that the expression level of AfSVP was significantly higher in flowers compared to other tissues (P<0.05). The analysis of high, low temperature and drought stress response showed that the expression of AfSVP reached the highest at 12 h under high temperature stress, at 72 h under low temperature stress and at 12 h under drought stress (P<0.05). This study offers a reference for further research on how plants adapt to environmental changes.
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Received: 28 April 2024
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Corresponding Authors:
**wangyqly@163.com
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| About author:: * These authors contributed equally to this work |
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