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| Effect of Mechanical Wounding on Jasmonic Acid Synthesis and Signaling at Potato (Solanum tuberosum) Tuber Wounds |
| BAI Lu, YANG Rui-Rui, LI Fang-Hong, WANG Ying, WANG Xue-Xue, ZHANG Xue-Jiao, HAN Ye, BI Yang* |
| College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China |
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Abstract Fruit and vegetables are highly susceptible to mechanical wounding during harvest and postharvest handling, and the wounds induce the production of several phytohormones at the wounds, but involvement of jasmonic acid (JA) in potato (Solanum tuberosum) wound stress has not been reported. In this study, based on transcriptome analysis of pre-wounded potato tubers, the genes related to JA synthesis and signaling were screened, the expression of these genes and related enzyme activities were analyzed,the JA content at the wounds was determined. The results showed that the wound significantly upregulated the expression levels of genes related to JA synthesis at the tuber wound, including lipoxygenase (LOX) family members StLOX1.5, StLOX2, StLOX3.1 and StLOX6, propadiene oxidative synthase (AOS) family members StAOS1, StAOS2 and StAOS3, and propadiene cyclohexylase (AOC) family members StAOC and 12-oxo-dienoic acid reductase (OPR) family members StOPR1, StOPR2 and StOPR3. The wound also significantly increased LOX and AOS activities and significantly increased JA levels at the tuber wounds. In addition, the wound significantly upregulated the expression levels of key JA signaling genes, the jasmonate ZIM domain (JAZ) family members StJAZ1, StJAZ2, StJAZ3, StJAZ4 and StJAZ7. In conclusion, the wound activates JA synthesis and signaling in potato tuber wounds. This study provides a theoretical basis for the study of the response mechanism of potato wound stress.
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Received: 27 March 2024
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Corresponding Authors:
* biyang@gsau.edu.cn
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