Identification of microRNA in 'Xinyu' Grape (Vitis vinifera) and Its Response Analysis to High Temperature Stress
NIU Li-Li6,*, WU Yun-Long1,*, LIU Li-Yuan1, SONG Yang-Bo3, CAO Hong-Yan2, DU Ting-Ting2, WANG Sheng-Jie2, XI Lin4, YANG Qing2, MENG Dong2, ZHOU Hui1,**, HE Kun-Zu5,**
1 Turpan Grape Industry Development Promotion Center, Turpan 838000, China; 2 College of Forestry, Beijing Forestry University, Beijing 100083, China; 3 College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China; 4 University of Hohenheim, Stuttgart, Germany; 5 Turpan Silu Mingzhu Agricultural Biotechnology Co., Ltd., Turpan 838000, China; 6 Institute of Medicinal Plant Development, China Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
Abstract High temperature stress is one of the primary abiotic stresses that restrict fruit tree production. Grape (Vitis vinifera) is a cultivated fruit crop with high economic value worldwide, and its growth and development are often affected by high temperature stress. Studying the response mechanism of grapes to high temperature stress is of great significance for understanding the domestication of grapes to high temperature stress. In this study, 'Xinyu' grape leaves were collected for high-throughput sequencing and small RNA (sRNA) libraries were constructed for the control group and the high-temperature stress treatment group, respectively. A series of miRNAs related to high temperature stress response were identified, and their functions in high temperature tolerance response were analyzed. A total of 171 known miRNAs and 251 new miRNAs were identified, among which 6 known miRNAs and 21 new identified miRNAs were differentially expressed under high temperature stress. A total of 297 target genes were predicted for these differentially expressed miRNAs. The expression of 10 selected miRNAs and their target genes was validated through qRT-PCR, indicating that these target genes had a significant response to high temperature stress. Gene function and pathway analysis indicated that these genes might play an important role in high temperature stress tolerance. The results of this study provide a theoretical basis for further understanding the high-temperature stress response during fruit tree domestication and the breeding of grape varieties resistant to high-temperature stress.
NIU Li-Li,WU Yun-Long,LIU Li-Yuan, et al. Identification of microRNA in 'Xinyu' Grape (Vitis vinifera) and Its Response Analysis to High Temperature Stress[J]. 农业生物技术学报, 2024, 32(9): 2009-2020.
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