MAX1 Gene Cloning and Its Response to High Temperature Stress in Green Onions (Allium fistulosum)
ZHANG Yu-Chen1,2, BAI Mi-Feng1,2, XU Huan-Huan2, XING Jia-Yi2, ZHOU Xu-Yang2, LIU Le-Cheng1,*, WANG Yong-Qin2,*
1 College of Horticulture, Yangtze University, Jingzhou 434025, China; 2 Institute of Vegetables, Beijing Academy of Agriculture and Forestry Sciences/State Key Laboratory of Vegetable Biological Breeding/National Engineering Technology Research Center/Beijing Key Laboratory of Vegetable Germplasm Improvement/Key Laboratory of Urban Agriculture in North China, Ministry of Agriculture and Rural Affairs, Beijing 100097, China
Abstract More axillary growth 1 (MAX1) is a key gene in the strigolactone synthesis pathway, which plays an important role in the tillering regulation of green onions (Allium fistulosum) and resistance to adversity stress. In this study, AfMAX1 gene (GenBank No. AfisC2G04224) was cloned from green onions, and bioinformatics analysis, subcellular localization, tissue expression patterns, and high-temperature stress response analysis were carried out. The results showed that the CDS of AfMAX1 was 1 545 bp long, encoded 514 amino acids, and had a relative molecular weight of 58.55 kD. AfMAX1 was a hydrophilic protein with no signal peptide and transmembrane domain. Protein sequence comparison analysis showed that MAX1 was conserved in multiple species. Subcellular localization results showed that AfMAX1 might be localized in the cytoplasm and nucleus of Nicotiana benthamiana. Expression pattern analysis showed that AfMAX1 had the highest expression level in the leaf sheath, followed by in the roots; The expression level of AfMAX1 significantly increased after 12 h of high temperature stress (P<0.01). The above results indicate that AfMAX1 could respond to high temperature stress, which might help enhance plant tillering regulation and heat tolerance. This study provides basic data for in-depth exploration of the molecular mechanisms of green onion plant type regulation and abiotic stress resistance.
ZHANG Yu-Chen,BAI Mi-Feng,XU Huan-Huan, et al. MAX1 Gene Cloning and Its Response to High Temperature Stress in Green Onions (Allium fistulosum)[J]. 农业生物技术学报, 2024, 32(8): 1776-1783.
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