Abstract The cytoplasmic male sterile line (CMS) is one of the important prerequisites for breeding superior hybrid rice (Oryza sativa) varieties, cultivating excellent CMS is of great significance for hybrid rice breeding. In this study, the high-resistant and high-quality rice variety 'Huahang Simiao' was used as the donor parent, and it was crossed with the Indica three-line maintainer line 'Fudao B', which had good comprehensive agronomic traits but poor grain quality and rice blast resistance. In the F3 generation, superior plants were selected as male parents and crossed with 'Gufeng A' as the female parent. The progeny from the crosses were continuously subjected to shuttle breeding and selection in Sanya and Shaxian bases. After multiple generations of elimination and selection, a stable CMS line named 'Funong A' was finally bred, and its corresponding maintainer line was named 'Funong B'. Comprehensive analysis of agronomic traits revealed that 'Funong A' had good leaf morphology, sturdy stems, and erect flag leaves, with a morning flowering ratio of 85%. The stem diameter and thickness of 'Funong A' were significantly larger than those of the parent lines. Field tests and microscopic observations showed that the sterility rate of 'Funong A' was 100%, pollen sterility ratio was 99.99%, and stigma exsertion rate was 57.12%. 'Funong A' exhibited a significantly larger basal internode diameter and thicker stem wall, as well as higher content of hemicellulose and silicon in stems. High-density chip analysis revealed the presence of semi-dwarf gene Sd-1, spikelet development-related gene qNGR9, and grain weight gene OsSPL16 in 'Funong A'. Additionally, Funong A successfully combined 3 rice blast resistance genes, Pi5, Pid2, and Pid3, with an indoor identification rate of resistant strains reaching 81.82%. Moreover, the 7 rice varieties developed using 'Funong A' exhibited high yield, good grain quality, and intermediate resistance to rice blast disease. It is evident that 'Funong A' possesses strong development potential and can provide support for the combination and breeding of hybrid rice with good resistance, high yield, and superior rice quality.
TU Shi-Hang,LIAN Ling,HONG Yong-He, et al. Breeding of the Three-line Indica Cytoplasmic Male Sterile Line 'Funong A' and Its Comprehensive Agronomic Trait Analysis[J]. 农业生物技术学报, 2024, 32(3): 701-711.
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