梯棱羊肚菌促生恶臭假单胞菌X14的分离鉴定

doi:10.3969/j.issn.1674-7968.2024.03.020

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Abstract Morel (Morchella spp.) is a world-famous rare edible and medicinal mushroom. In the artificial cultivation process morels must be covered with soil to produce fruit. At present, the mechanism of soil microorganisms affecting the growth and development of morel is still unknown. 132 strains of bacteria were isolated and purified from morel soil. Through the plate confrontation culture test, it was found that 15 strains had an antagonistic effect on the hyphae of Morchella importuna and 4 strains had the effect of promoting growth. Among them, when strain X14 was co-cultured with morel on plate for 4 and 8 d, the growth rate of morel hyphae was significantly increased by 7.6% and 11.6% compared with the control (P<0.05). Morel and strain X14 were inoculated into wheat medium in test tubes and co-cultured. The growth rate of morel hyphae was measured at different culture time. The results showed that the growth rate of morel hyphae treated by strain X14 was very extremely significantly increased by 28.3%, 24.8% and 24.0% compared with the control at 4, 6 and 7 d, respectively (P<0.001). Morel and strain X14 were inoculated into stock flasks and co-cultured. The results showed that the growth rate of morel hyphae treated by strain X14 was not significantly different from the control at 5 d of co-culture (P>0.05). After 10 d of co-cultivation, the growth rate of morel hyphae treated with strain X14 was extremely significantly increased by 14.1% compared with the control (P<0.01). The culture period of the stock seed was shortened by 4~6 d compared with the control. Based on the comprehensive analysis of colony morphology, physiological and biochemical characteristics and 16S rDNA sequence, the strain X14 was identified as Pseudomonas putida. In this study, a strain of Pseudomonas putida X14 was screened from the soil of morel, which could promote the hyphae growth of Morchella importuna and provide technical support for the development of microbial agents that promote the growth of morel.

Key words： Morchella importuna Pseudomonas putida Mycelial growth-promoting Screening of microorganisms

Received: 10 November 2022

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Corresponding Authors: * xujunsea@163.com

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	LEI Xiao
	ZHOU Bing-Yu
	ZHOU Yu
	TANG Shao-Jun
	YANG Di
	REN Rui
	MA Wen-Jie
	MA Wen-Hua
	SHAO Chen-Xia
	YANG Yi
	XU Jun

Cite this article:

LEI Xiao,ZHOU Bing-Yu,ZHOU Yu, et al. Isolation and Identification of Morchella importuna Growth-promoting Pseudomonas putida X14[J]. 农业生物技术学报, 2024, 32(3): 712-719.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2024.03.020 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2024/V32/I3/712

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