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| The Effect of miR-133a_R+1 Regulation to ALDH5A1 Expression on Retained Placenta in Cows (Bos taurus) |
| WANG Yue, LYU Chen, YAO Dan, ZHAO Xing-Xu*, ZHANG Yong* |
| Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation/College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China |
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Abstract Micro RNA (miRNA) is involved in numerous physiological activities in mammals, with miR-133a playing a pivotal regulatory role in placental and uterine growth and development. miR-133a_R+1 and ALDH5A1 are differentially expressed in placental tissues of cows (Bos taurus) with retained placenta and may have a potential targeting regulatory relationship through sequencing and target gene prediction software. In this study, normal fetal placental tissue, RP placental tissue, and bovine endometrial epithelial cells (BEND) were used as experimental materials. Hematoxylin eosin (HE) staining was used to observe the pathological changes of placental tissue, and qRT-PCR was used to detect expression of miR-133a_R+1 and aldehyde dehydrogenase 5 family member A1 (ALDH5A1) in placental tissue. Immunohistochemistry (IHC) was used to detect the distribution of ALDH5A1 protein in placental tissue. Optimal transfection conditions was 100 nmol/L 133a_R+1 mimic for 12 h to establish a BEND cell model overexpressing miR-133a_R+1. qRT-PCR and Western blot were used to detect the expression of ALDH5A1 gene and its protein in the cells. The results showed that RP placental tissue villi were short, sparse, and fragmented. The expression level of miR-133a_R+1 mRNA in RP placenta tissue was extremely significantly up-regulated (P<0.01), ALDH5A1 was expressed in connective tissue and cytotrophoblast, and the expression level was extremely significantly down-regulated (P<0.01). The expression levels of ALDH5A1 gene and its protein in BEND cells transfected with miR-133a_R+1 mimic were extremely significantly downregulated (P<0.01). In summary, miR-133a_R+1 affected cow RP by targeting the expression of ALDH5A1 gene and its protein. This study provides corresponding theoretical basis for further exploring the mechanism of miRNA action on dairy cow RP.
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Received: 04 December 2023
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Corresponding Authors:
*zhaoxx@gsau.edu.cn;zhychy@163.com
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