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| Effects of Low Temperature on Antioxidant Capacity and Lipid Metabolism in the Liver of Hezuo Pig (Sus scrofa) |
| JIA Rui, LI Yao, WANG Long-Long, WANG Peng-Fei, YAN Zun-Qiang, ZHANG Peng-Xia, YANG Qiao-Li* |
| College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China |
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Abstract Low temperature is one of the factors affecting the efficiency of pig production in the northern region of China, which leads to the decline of pig production performance and the increase of piglet disease and death rate, and seriously restricts the efficient development of China's pig industry. The aim of this study was to analyzing the effects of low temperature on liver antioxidant capacity and lipid metabolism in Hezuo pigs (Sus scrofa). 75-day-old healthy Hezuo pigs were selected and randomly divided into low-temperature group (CH) and normothermic control group (NH) after 7 d of room temperature rearing. The CH was reared in a room equipped with a temperature control device (-15±2) ℃, and the NH was reared in a room at temperature (23±2) ℃. The precess period was 15 d. Blood was collected from fasting anterior vena cava on the 0, 5, 10, and 15 d of the precess period, and liver tissues were collected from slaughter on the 15 d; serum antioxidant indexes and hepatic lipid metabolites were detected by ELISA, and lipid metabolism-related genes were detected by qPCR. The results showed that with the prolongation of low temperature treatment time, the serum total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content of Hezuo pigs showed a trend of increasing and then decreasing, and the T-AOC activity reached the highest value of 1.94 on 10 d, which was significantly higher than the other treatment (P<0.05), and the MDA activity was the highest on 5 d, which was significant higher than the 10 and 15 d (P<0.05), The activities of serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) showed an increasing trend, in which SOD was extremely significant (P<0.01) after 10 d of low temperature treatment, GSH-Px and CAT activities were extremely significant (P<0.01) after 5 d of low temperature treatment. The contents of protein carbonyl (PCO) and 8-hydroxy-2deoxyguanosine (8-OHdG), markers of oxidative damage in pig livers, were extremely significantly elevated (P<0.01) in the CH compared with the NH. The activities of fatty acid synthase (FAS) and acetyl CoA carboxylase (ACC), the enzymes related to lipid metabolism, were significantly reduced (P<0.05) in pig livers exposed to low temperature. The activities of lipoprotein lipase (LPL), hepaticlipase (HL), alanine aminotransferase (ALT), and stearoyl-CoA desaturase 1 (SCD1) were all significantly reduced (P<0.01); carnitine palmitoyltransferase enzyme (CPT) activity was extremely significantly elevated (P<0.01). The serum triglyceride (TG) content of the low-temperature Hezuo pigs was the highest at 0.82 on 5 d, and significantly decreased to 0.61 on 15 d (P<0.05). The expression of hepatic lipid metabolism-related genes sterol regulatory element-binding protein-1c (SREBP-1c) and peroxisome proliferators-activated receptors α (PPARα) was significantly reduced (P<0.05), and p300/CBP associated factor (PCAF) expression was extremely significantly increased (P<0.01). In summary, the low temperature caused oxidative damage to the liver of Hezuo pigs, and Hezuo pigs could improve their cold adaptation by enhancing hepatic lipid metabolism when they were in a cold environment. This study provides a reference basis for further exploring the mechanism of the formation of cold-resistant traits in Hezuo pigs.
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Received: 03 November 2023
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Corresponding Authors:
*yangql0112@163.com
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