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| The Relationship Between Rhizosphere Soil Fungal Communities, Nutrients and Growth Performance of Torreya dapanshanica seedlings |
| FENG Li-Zhi1, CHEN Hai-Qing2,3, TENG Qiu-Mei1, LYU Qiang-Feng2,3, LI Shu-Min1, ZHANG Qian-Qian1,3, DU Jian-Hang1, LI Yong-Chun1,3, YU Ye-Fei2,3,* |
1 College of Environmental and Resource, College of Carbon Neutrality, Zhejiang A&F University, Hangzhou 311300, China;
2 Dapanshan National Nature Reserve Administration of Zhejiang Province, Jinhua 322300, China;
3 Zhejiang Dapanshan Forest Ecosystem Research and Observation Station, Jinhua 322300, China |
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Abstract Rhizosphere fungi play an important role in helping plants absorb nutrients and maintaining normal growth of trees. To clarify the relationship between the rhizosphere fungal community, nutrient limitation and seedling growth of Torreya dapanshanica,the artificially cultivated T. dapanshanica of different growth performance was subjected to the research. High-throughput sequencing technology was used to determine the composition and trophic modes of the rhizosphere soil fungal community of T. dapanshanica sapling with different growth performance. Meanwhile, the physicochemical properties of the rhizosphere soil and the stoichiometric characteristics of soil enzymes of the two kinds of soil were analyzed. The results showed that: (1) The amounts of soil organic carbon, ammonium nitrogen, available phosphorus, and total phosphorus in the rhizosphere of advantaged trees were significantly higher than those of disadvantaged trees. Analysis of soil enzyme stoichiometric ratios revealed that disadvantaged trees were subjected to nitrogen limitation. (2) The Chao1 index and Shannon index of the rhizosphere soil fungal community in advantaged trees were significantly higher than that in disadvantaged trees. And non-metric multidimensional scaling (NMDS) analysis showed that the rhizosphere soil fungal compositions of T. dapanshanica saplings with different growth performance could be clearly separated. (3) The results of fungal trophic modes analysis indicated that, compared with the rhizosphere of disadvantaged trees, the relative abundance of symbiotrophic fungi in the rhizosphere soil of advantaged trees was significantly higher, while the relative abundance of pathotrophic fungi was significantly lower. Moreover, plant height was significantly positively correlated with the relative abundance of rhizosphere symbiotrophic fungi and significantly negatively correlated with the relative abundance of pathotrophic fungi. (4) Canonical correspondence analysis showed that the main factors affecting the rhizosphere fungal composition of advantaged trees were soil ammonium nitrogen and available phosphorus contents, and the rhizosphere fungal composition was positively correlated with soil phosphatase activity; whereas the rhizosphere fungal community of disadvantaged trees was greatly affected by nitrogen limitation. In summary, the advantaged trees had a higher abundance of symbiotic fungi in the rhizosphere and a more adequate supply of phosphorus, which was conducive to maintaining their good tree performance; The abundance of pathotrophic fungi in the rhizosphere soil of disadvantaged trees was relatively high, with the presence of soil nitrogen limitation. Therefore, the combined application of nitrogen fertilizer and organic fertilizer could alleviate the rhizosphere nutrient limitation of disadvantaged trees and reduce the abundance of pathotrophic fungi. This study provides a basis for maintaining the good growth and conservation of rare plants through rhizosphere regulation under adverse site conditions.
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Received: 06 August 2025
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Corresponding Authors:
*feieryun1126@126.com
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[1] 安志刚, 郭凤霞, 陈垣, 等. 2018. 连作自毒物质与根际微生物互作研究进展[J]. 土壤通报, 49(3): 750-756.
(An Z G, Guo F X, Chen Y, et al.2018. Advances in interactions between autotoxic compounds andrhizosphere microbes in continuous cropping obstacles[J]. Chinese Journal of Soil Science, 49(3): 750-756.)
[2] 陈懂懂, 霍莉莉, 赵亮, 等. 2023. 青海高寒草地水热因子对土壤微生物生物量碳、氮空间变异的贡献—基于增强回归树模型[J]. 生态环境学报, 32(07): 1207-1217.
(Chen D D, Huo L L, Zhao L, et al.2023. Contributionof water and heat factors to spatial variability of soil microbial biomass carbon and nitrogen in oinghai alpine grassland: Based onenhanced regression tree model[J]. Ecology and Environmental Sciences, 32(07): 1207-1217.)
[3] 陈祖静, 高尚坤, 陈园, 等. 2020. 短期施肥对桉树人工林土壤真菌群落结构及功能类群的影响[J]. 生态学报, 40(11): 3813-3821.
(Chen Z J, Gao S K, Chen Y, et al.2020. Effects of short-term fertilization on soil fungal community structure andfunctional group in Eucalyptus artificial forest[J]. Acta Ecologica Sinica, 40(11): 3813-3821.)
[4] 方宇, 白涛, 刘冬梅, 等. 2022. 烟草黑胫病植株根际土壤真菌群落多样性及结构分析[J]. 西南农业学报, 35(04): 822-830.
(Fang Y, Bai T, Liu D M, et al.2022. Diversity and structure of the fungal community in rhizosphere soil of tobacco plants with black shank disease[J]. Southwest China Journal of Agricultural Sciences, 35(04): 822-830.)
[5] 龚金玉, 彭金根, 谢利娟, 等. 2021. 深圳梧桐山不同树势毛棉杜鹃根际土壤微生物多样性分析[J]. 林业科学, 57(11): 190-200.
(Gong J Y, Peng J G, Xie L J, et al.2021. Microbial diversity in rhizosphere soil of rhododendron moulmainense with different tree potential in wutong mountain of Shenzhen[J]. Scientia Silvae Sinicae, 57(11): 190-200.)
[6] 黄澳, 及利, 何功秀, 等. 2025. 杉木闽楠混交林根际土壤生态化学计量特征及其根际效应[J]. 生态学杂志, 44(7): 2268-2276.
(Huang A, Ji L, He G X, et al. 2025. Ecological stoichiometric characteristics and their rhizosphere effects of soil in mixed forests of Cunninghamia lanceolata and Phoebe bournei[J]. Chinese Journal of Ecology, (7): 2268-2276.)
[7] 靳莉雅, 张志明, 刘文杰, 等. 2025. 西双版纳典型森林转变对土壤酶活性及微生物养分限制的影响[J]. 生态学杂志, 44(7): 2259-2267.
(Jin L Y, Zhang Z M, Liu W J, et al.2025. Effects of typical forest transformation on soi enzvme activity and microbial nutrient limitations in tropica xishuangbanna, china[J]. Chinese Journal of Ecology, 44(7): 2259-2267.)
[8] 句娇, 李迎超, 王利兵, 等. 2020. 水肥耦合效应对栓皮栎苗木生长的影响[J]. 浙江农林大学学报, 37(04): 673-682.
(Ju J, Li C Y, Wang L B, et al.2020. Coupling effects of soil water and fertilizer application on the growth of Quercus variabilis seedlings[J]. Journal of Zhejiang A&F University, 37(04): 673-682.)
[9] 刘谣, 刘金超, 宋钰珑, 等. 2023. 季节变化对川西亚高山森林土壤酶活性及化学计量特征的影响[J]. 四川农业大学学报, 41(03): 456-463.
(Liu Y, Liu J C, Song Y L, et al.2023. Effects of seasonal changes on soil enzyme activities and their stoichiometric characteristics of subalpine forests in western sichuan[J]. Journal of Sichuan Agricultural University, 41(03): 456-463.)
[10] 刘振花. 2019. 不同氮肥施用对杨树栽培土壤改良效果分析[J]. 防护林科技, (02): 38-40.
(Liu Z H. 2019. Effect of different fertilization treatments on soil improvement of poplar forest land[J]. Protection Forest Science and Technology, (02): 38-40.)
[11] 彭丽鸿, 崔朝伟, 王佳琪, 等. 2022. 连栽对杉木人工林土壤表层真菌群落结构及功能的影响[J]. 福建农林大学学报(自然科学版), 51(05): 621-628.
(Peng L H, Cui C W, Wang J Q, et al.2022. Effects of continuous planting on the structure and function of soil fungi community in chinese fir plantation[J].Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 51(05): 621-628.)
[12] 唐铭灿, 王香香, 缪怡, 等. 2025. 不同种植模式下微生物养分限制调节土壤有机碳积累[J].农业资源与环境学报, 42(02): 349-359.
(Tang M C, Wang X X, Miao Y, et al.2025. Characteristics of microbial nutrient limitation regulate soil organie carbon sequestration in different crop-planting patterns[J]. Journal of Agricultural Resources and Environment, 42(02): 349-359.)
[13] 佘佳荣, 郑琼, 张轩, 等. 2023. 湘西地区珍稀树木根际微生物群落结构及土壤理化性质研究[J]. 中南林业科技大学学报, 43(12): 104-115.
(She J R, Zheng Q, Zhang X, et al.2023. Study on rhizosphere microbial community structure and soil physical and chemical properties of rare trees in xiangxi region[J]. Journal of Central South University of Forestry & Technology, 43(12): 104-115.)
[14] 王传盈, 王凯月, 王浩然, 等. 2024. 黄河下游典型湿地土壤养分及其生态化学计量特征[J]. 环境科学, 45(03): 1674-1683.
(Wang C Y, Wang K Y, Wang H R, et al.2024. Nutrients and ecological stoichiometry characteristies of typical wetland soils in the lower yellow river[J]. Environmental Science, 45(03): 1674-1683.)
[15] 王汉之, 马小明, 方雪梅, 等. 2025. 入侵植物银胶菊对土壤酶活性及微生物养分限制的影响[J]. 草地学报, 33(01): 70-78.
(Wang H Z, Ma X M, Fang X M, et al.2025. The effects of invasion plant Parthenium hysterophorus on soil enzyme activity and microbial nutrient limitation[J]. Acta Agrestia Sinica, 33(01): 70-78.)
[16] 汪其同, 高明宇, 刘梦玲, 等. 2017. 基于高通量测序的杨树人工林根际土壤真菌群落结构[J]. 应用生态学报, 28(04): 1177-1183.
(Wang Q T, Gao M Y, Liu M L, et al.2017. Illumina miseq sequencing-based fungal community of rhizosphere soils along root orders of poplar plantation[J]. Chinese Journal of Applied Ecology, 28(04): 1177-1183.)
[17] 王小河, 辜夕容, 李杰, 等. 2020. 榧树属植物的无性繁殖技术研究进展[J]. 生物技术通报, 36(12): 178-187.
(Wang X H, Gu X R, Li J, et al.2020. Review on vegetative propagation techniques of Torreyaarn[J]. Biotechnology Bulletin, 36(12): 178-187.)
[18] 杨慧琴, 向涌旗, 吕倩, 等. 2024. 3种混交林造林初期土壤真菌群落结构特征[J]. 生态学报, 44(08): 3360-3371.
(Yang H Q, Xiang Y Q, Lyu Q, et al.2024. A comparative study on the soil fungal community structure across three mixed forests at the initial stage of afforestation[J]. Acta Ecologica Sinica, 44(08): 3360-3371.)
[19] 尤垂淮, 高峰, 王峰吉, 等. 2015. 连作对云南烤烟根际微生态及烟叶产质量的影响[J]. 中国烟草学报, 21(01): 60-67.
(You C H, Gao F, Wang F J, et al.2015. Effect of continuous cropping on rhizosphere micro-ecology as well as on yield and quality of flue-cured tobacco in yunnan[J]. Acta Tabacaria Sinica, 21(01): 60-67.)
[20] 张英, 曹红雨, 赵珮杉, 等. 2025. 半干旱-亚湿润干旱区樟子松林地土壤真菌群落随林龄增长的演变[J]. 环境科学, 46(6): 3975-3984.
(Zhang Y, Cao H Y, Zhao P S, et al.2025. Evolution of soil fungal community with the stand aging of Pinus sylvestris var. mongolica forests in semi-arid and dry sub-humid regions[J]. Environmental Science, 46(6): 3975-3984.)
[21] 朱文娟, 任月梅, 杨忠, 等. 2023. 谷子土壤微生物群落结构及功能预测分析[J]. 作物杂志, (05): 170-178.
(Zhu W J, Ren Y M, Yang Z, et al. 2023. Structure and predicted functional analysis of microbial community of millet soil[J]. Crops, (05): 170-178.)
[22] Boring T J, Thelen K D, Board J E, et al.2018. Phosphorus and potassium fertilizer application strategies in corn-soybean rotations[J]. Agronomy, 8(9): 195.
[23] Cao M, Liu R, Xiao Z, et al.2022. Symbiotic fungi alter the acquisition of phosphorus in Camellia oleifera through regulating root architecture, plant phosphate transporter gene expressions and soil phosphatase activities[J]. Journal of Fungi, 8(8): 800.
[24] Chen D, Zhang Y, Lin Y, et al.2010. Changes in belowground carbon in Acacia crassicarpa and Eucalyptus urophylla plantations after tree girdling[J]. Plant and Soil, 326: 123-135.
[25] Chen Z, Li Y, Chang S X, et al.2021. Linking enhanced soil nitrogen mineralization to increased fungal decomposition capacity with moso bamboo invasion of broadleaf forests[J]. Science of the Total Environment, 771: 144779.
[26] Cheng B, Liu H, Bai J, et al.2022. Soil fungal composition drives ecosystem multifunctionality after long-term field nitrogen and phosphorus addition in alpine meadows on the tibetan plateau[J]. Plants, 11(21): 2893.
[27] Fang M, Liang M, Liu X, et al.2020. Abundance of saprotrophic fungi determines decomposition rates of leaf litter from arbuscular mycorrhizal and ectomycorrhizal trees in a subtropical forest[J]. Soil Biology and Biochemistry, 149: 107966.
[28] Fang W, Song Z, Tao S, et al.2020. Biochar mitigates the negative effect of chloropicrin fumigation on beneficial soil microorganisms[J]. Science of the Total Environment, 738: 139880.
[29] Guo X, Yang Y, Niu Z, et al.2019. Characteristics of microbial community indicate anthropogenic impact on the sediments along the yangtze estuary and its coastal area, China[J]. Science of the Total Environment, 648: 306-314.
[30] Li Y, Han X, Li B, et al.2023. Soil addition improves multifunctionality of degraded grasslands through increasing fungal richness and network complexity[J]. Geoderma, 437: 116607.
[31] Li Y, Li Y, Chang S X, et al.2017. Linking soil fungal community structure and function to soil organic carbon chemical composition in intensively managed subtropical bamboo forests[J]. Soil Biology and Biochemistry, 107: 19-31.
[32] Li Y, Liu B, Li S, et al.2014. Shift in abundance and structure of soil ammonia-oxidizing bacteria and archaea communities associated with four typical forest vegetations in subtropical region[J]. Journal of Soils and Sediments, 14: 1577-1586.
[33] Lu Y, Chen Z, He A, et al.2022. Torreya dapanshanica (Taxaceae), a new species of gymnosperm from Zhejiang, east China[J]. Phytokeys, 192: 29.
[34] Yin Y, Yuan Y, Zhang X., et al.2022. Comparison of the responses of soil fungal community to straw, inorganic fertilizer, and compost in a farmland in the Loess Plateau[J]. Microbiology Spectrum, 10(1): e02230-21.
[35] Nguyen N H, Song Z, Bates S T, et al.2016. Funguild: An open annotation tool for parsing fungal community datasets by ecological guild[J]. Fungal Ecology, 20: 241-248.
[36] Shi Z, Mickan B, Feng G, et al.2015. Arbuscular mycorrhizal fungi improved plant growth and nutrient acquisition of desert ephemeral Plantago minuta under variable soil water conditions[J]. Journal of Arid Land, 7: 414-420.
[37] Sinsabaugh R L, Lauber C L, Weintraub M N, et al.2008. stoichiometry of soil enzyme activity at global scale[J]. Ecology Letters, 11(11): 1252-1264.
[38] Smith SE, Smith FA.2011. Roles of arbuscular mycorrhizas in plant nutrition and growth: New paradigms from cellular to ecosystem scales[J]. Annual Review of Plant Biology, 62(1): 227-250.
[39] Tedersoo L, Bahram M, Cajthaml T, et al.2016. Tree diversity and species identity effects on soil fungi, protists and animals are context dependent[J]. The Isme Journal, 10(2): 346-362.
[40] Tauro T P, Mtambanengwe F, Mpepereki S, et al.2021 Soil fungal community structure and seasonal diversity following application of organic amendments of different quality under maize cropping in Zimbabwe[J]. PLOS ONE, 16(10): e0258227.
[41] Wang C, Wan S, Xing X, et al.2006. Temperature and soil moisture interactively affected soil net n mineralization in temperate grassland in northern china[J]. Soil Biology and Biochemistry, 38(5): 1101-1110.
[42] Waring B G, Weintraub S R, Sinsabaugh R L.2014. Ecoenzymatic stoichiometry of microbial nutrient acquisition in tropical soils[J]. Biogeochemistry, 117: 101-113.
[43] Wen Y C, Li H Y, Lin Z A, et al.2020. Long-term fertilization alters soil properties and fungal community composition in fluvo-aquic soil of the North China Plain[J]. Scientific Reports, 10(1): 7198.
[44] Xiao L, Liu G, Li P, et al.2020. Ecoenzymatic stoichiometry and microbial nutrient limitation during secondary succession of natural grassland on the Loess Plateau, China[J]. Soil and Tillage Research, 200: 104605.
[45] Zhu J, Qu B, Li M.2017. Phosphorus mobilization in the yeyahu wetland: Phosphatase enzyme activities and organic phosphorus fractions in the rhizosphere soils[J]. International Biodeterioration & Biodegradation, 124: 304-313. |
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