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| Research Advances of Ultra-low Temperature Cryopreservation in Poultry Germplasm Resources |
| SONG Yong-Kang1, ZHANG Zhuo-Yuan1, CAI Liang-Yu1, WANG Lan-Ruo1, YAN Hai-Feng2, CAO Zu-Bing1,* |
1 College of Animal Science and Technology/Anhui Provincial Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, Anhui Agricultural University, Hefei 230036, China;
2 Hunan Institute of Animal and Veterinary Science, Changsha 410131, China |
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Abstract Ultra-low temperature cryopreservation is essential for establishing a poultry germplasm bank. The establishment of poultry germplasm cryobanks faces multiple challenges, including the inherent cryosensitivity of spermatozoa, the inability to successfully cryopreserve oocytes, reduced post-thaw viability, immature genetic material resuscitation technologies, and limited survival of offspring. Currently, ultra-low temperature cryopreservation technologies for poultry genetic resources remain underdeveloped, with core issues such as ultra-low temperature cryopreservation efficacy and underlying freezing mechanisms requiring further in-depth investigation. In this paper, advances in the cryopreservation of semen, somatic cells, primordial germ cells (PGCs) and gonadal tissues of poultry were reviewed, and the findings were summarized to provide a reference for the conservation of poultry germplasm resources.
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Received: 23 October 2025
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Corresponding Authors:
*zubingcao@ahau.edu.cn
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