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| Analysis of Imprinting and Methylation Status of the Bovine (Bos taurus) WWOX Gene |
| CHEN Wei-Na1,2,*, MA Chao3,*, LIANG Xiao-He2, ZHANG Yin-Jiao2, ZHENG Yun-Chang2, HOU Rui-Lin2, ZHANG Cui2, LI Shi-Jie2,** |
1 College of Traditional Chinese Medicine, Hebei University, Baoding 071000, China;
2 College of Life Science, Hebei Agricultural University, Baoding 071001, China;
3 Baoding No.2 Hospital, Baoding 071000, China |
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Abstract Imprinted genes play key roles in embryonic and placental development, animal growth, and the process of disease development. Imprinted genes are usually found in clusters on the genome. The WW domain-containing oxidoreductase (WWOX) gene is located 2.97 Mb upstream of the CDH13-GSE1 imprinted region on chromosome 18 in cattle (Bos taurus) and encodes a WW domain-containing oxidoreductase. To analyze the imprinting status of the WWOX gene in cattle, in this study, the imprinting status of the WWOX gene in tissues (heart, liver, spleen, lung, kidney and brain) and placenta of wild type and somatic cloned cattle was first analyzed using SNP-based approach. Based on a SNP locus (A/G, rs380382156) on exon 7, the WWOX gene was found to be biallelic expression in wild type bovine tissues and monoallelic expression in the placenta. WWOX gene was identified as a paternally imprinted gene in the placenta by analyzing the parental genotypes. WWOX gene also showed biallelic expression in the tissues of cloned cattle, whereas in the placenta of cloned cattle it showed biallelic expression. Methylation status was subsequently examined in the heart, lung and placenta of wild type and cloned cattle. There was a differential methylation region (DMRs) found on intron 4 of the WWOX gene in the placenta, while it was hypermethylated in the heart and lung of wild type cattle. In cloned cattle, the WWOX gene exhibited a hypermethylation state across multiple organs (heart, lung, and placenta). The absence of its differentially methylated region suggested that DNA methylation participates in regulating the placenta-specific imprinted expression of this gene in wild-type cattle, while the aberrant methylation in the placenta of cloned cattle led to the disruption of this imprinted expression. This study provides a theoretical basis for further analyzing the role of imprinted genes in epigenetic reprogramming of donor nuclear in cloned animals and improving cloning efficiency.
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Received: 14 March 2025
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Corresponding Authors:
**lishijie20005@163.com
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| About author:: * These authors contributed equally to this work |
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