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| Identification of Four Viral Diseases in Melon (Cucumis melo) and Establishment of Multiplex Detection System |
| HAN Song-Lin*, LI Yao*, YANG Yu, WANG De-Fu, DU Jiang**, NIU Yan-Bing** |
| College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China |
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Abstract Melon (Cucumis melo) is an annual trailing herbaceous plant that plays a significant role in the agricultural industry of the Taigu district in Shanxi province. In recent years, viral diseases have severely impacted melon production in this region. To identify the pathogens responsible for these viral diseases and to establish rapid detection techniques, this study collected symptomatic melon samples from Taigu district that exhibited spots, mosaic patterns, shrinkage, and necrosis. These samples were analyzed comprehensively using RT-PCR combined with bioinformatics. The results indicated that the presence of quadruple viral pathogens in melo specimens, specifically for: Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV), Cucurbit aphid-borne yellows virus (CABYV), and Watermelon mosaic virus (WMV). Mixed infections were found to be prevalent. Based on homology comparisons and phylogenetic analyses of the coat protein (CP) sequences of the viruses mentioned above, the results indicated that the amplified isolates obtained in this study CABYV isolate Fenghuang, CMV isolate Fengyun, CGMMV isolate Longquan, and WMV isolate Zhouquan exhibited sequence identities of over 99.5%, 98.0%, 99.6%, and 97.0%, respectively, when compared with corresponding virus sequences deposited in the GenBank database. Each isolate had a relatively close genetic relationship with the corresponding isolate of the same virus. In response to the phenomenon of complex infection of sweet melon virus diseases in the local area, a multiplex RT-PCR detection system was established to simultaneously detect CMV, CGMMV, CABYV and WMV. Multiple RT-PCR amplification was performed on materials with complex infection of 4 viruses, resulting in 4 specific bands of 1 106, 690, 521, and 400 bp, respectively. The multiplex RT-PCR detection system developed in this study allows for rapid identification of melon viral diseases and provides technical support for the monitoring and prevention of melon virus diseases.
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Received: 14 March 2025
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Corresponding Authors:
** Corresponding authors, dujiang20080803@163.com;niuyanbingbest@163.com
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| About author:: * Thses authors contributed equally to this work |
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